使用蓝色葡聚糖二乙氨基乙基纤维素和嗜硫吸附剂从腹水液中通过快速蛋白质液相色谱法纯化小鼠单克隆抗体。
FPLC purification of mouse monoclonal antibodies from ascitic fluid using blue DEAE and thiophilic sorbents.
作者信息
Juronen E, Parik J, Toomik P
机构信息
Institute of General and Molecular Pathology, Tartu University, Estonia, U.S.S.R.
出版信息
J Immunol Methods. 1991 Jan 24;136(1):103-9. doi: 10.1016/0022-1759(91)90255-e.
Two fast methods for the purification of mouse monoclonal antibodies from ascites, fluids using Blue-DEAE and 'thiophilic' adsorbent (T gel) in the FPLC system are described. Blue-DEAE chromatography is useful only for IgG1, IgG2a and IgG2b monoclonal antibody purification. T gel is a satisfactory adsorbent for IgG2b purification. Other IgG subclasses and IgM can also be obtained by simple one-step procedures, but the preparations contain small amount of high molecular weight contaminants.
本文描述了两种在FPLC系统中使用Blue-DEAE和“嗜硫”吸附剂（T凝胶）从腹水、液体中快速纯化小鼠单克隆抗体的方法。Blue-DEAE色谱仅适用于IgG1、IgG2a和IgG2b单克隆抗体的纯化。T凝胶是用于纯化IgG2b的一种令人满意的吸附剂。其他IgG亚类和IgM也可通过简单的一步法获得，但制备物中含有少量高分子量污染物。
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