Juronen E, Parik J, Toomik P
Institute of General and Molecular Pathology, Tartu University, Estonia, U.S.S.R.
J Immunol Methods. 1991 Jan 24;136(1):103-9. doi: 10.1016/0022-1759(91)90255-e.
Two fast methods for the purification of mouse monoclonal antibodies from ascites, fluids using Blue-DEAE and 'thiophilic' adsorbent (T gel) in the FPLC system are described. Blue-DEAE chromatography is useful only for IgG1, IgG2a and IgG2b monoclonal antibody purification. T gel is a satisfactory adsorbent for IgG2b purification. Other IgG subclasses and IgM can also be obtained by simple one-step procedures, but the preparations contain small amount of high molecular weight contaminants.
本文描述了两种在FPLC系统中使用Blue-DEAE和“嗜硫”吸附剂（T凝胶）从腹水、液体中快速纯化小鼠单克隆抗体的方法。Blue-DEAE色谱仅适用于IgG1、IgG2a和IgG2b单克隆抗体的纯化。T凝胶是用于纯化IgG2b的一种令人满意的吸附剂。其他IgG亚类和IgM也可通过简单的一步法获得，但制备物中含有少量高分子量污染物。
