血清淀粉样蛋白A（SAA）蛋白的固相酶免疫测定法。临床评估。

A solid phase enzyme immunoassay for serum amyloid A (SAA) protein. Clinical evaluation.

作者信息

Taktak Y S, Lee M

机构信息

Department of Chemical Pathology, Leeds University, U.K.

出版信息

J Immunol Methods. 1991 Jan 24;136(1):11-6. doi: 10.1016/0022-1759(91)90243-9.

DOI:10.1016/0022-1759(91)90243-9

PMID:1995703

Abstract

An enzyme-linked immunosorbent assay for serum amyloid A protein using commercially available antisera has been developed. Immulon II microtitre plates are coated with undenatured sera by overnight incubation at 4 degrees C. The assay combines the advantages of simplicity, rapidity, precision, sensitivity and safety and confirms the suitability of measuring serum amyloid A protein as a sensitive marker during the acute-phase response. This method permits the rapid processing of a large number of samples.

摘要

已开发出一种使用市售抗血清检测血清淀粉样蛋白A的酶联免疫吸附测定法。Immulon II微量滴定板在4℃下过夜孵育，用未变性血清包被。该测定法结合了简单、快速、精确、灵敏和安全的优点，并证实了将血清淀粉样蛋白A作为急性期反应期间的敏感标志物进行测量的适用性。该方法能够快速处理大量样本。