Matthiesen Rune, Carvalho Ana Sofia
Instituto de Patologia e Imunologia Molecular da Universidad do Porto - IPATIMUP, Porto, Portugal.
Methods Mol Biol. 2010;593:187-204. doi: 10.1007/978-1-60327-194-3_10.
Protein quantitation by mass spectrometry (MS) is attractive since it is possible to obtain both the identification and quantitative values of novel proteins and their posttranslational modifications in one experiment. In contrast, protein arrays only provide quantitative values of targeted proteins and their modifications. There are an overwhelming number of quantitative mass spectrometry (MS) methods for protein and peptide quantitation. The aim here is to provide an overview of the most common MS-based quantitative methods used in the proteomics field and discuss the computational algorithms needed for the robust quantitation of proteins, peptides, and their posttranslational modifications.
通过质谱（MS）进行蛋白质定量很有吸引力，因为在一次实验中就有可能获得新蛋白质及其翻译后修饰的鉴定和定量值。相比之下，蛋白质阵列仅提供目标蛋白质及其修饰的定量值。有大量用于蛋白质和肽定量的定量质谱（MS）方法。本文旨在概述蛋白质组学领域中最常用的基于质谱的定量方法，并讨论对蛋白质、肽及其翻译后修饰进行可靠定量所需的计算算法。
