Division of Functional Histology, Department of Functional Biomedicine, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime 791-0295, Japan.
Phytother Res. 2010 Jul;24(7):995-1003. doi: 10.1002/ptr.3048.
Olive (Olea europaea L.) leaves have long been used in folk medicine and herbal tea in Europe and the Mediterranean area. The Mediterranean climate is characterized by high temperatures, and by strong ultraviolet B (UVB) radiation causing the skin to age, increasing wrinkling, pigmentation and skin thickness. The aim of this study was to examine the effects of an olive leaf extract and its component oleuropein on skin damage caused by acute UVB irradiation in C57BL/6J mice. The extract (300 or 1000 mg/kg) and oleuropein (25 or 85 mg/kg) were administered orally twice daily for 14 days. UVB was administered daily at a dose of 120 mJ/cm(2) for the first 5 days and then every other day for 9 days. Both treatments inhibited the increases in skin thickness induced by radiation. They also inhibited increases in the Ki-67- and 8-hydroxy-2'-deoxyguanosine-positive cell numbers, melanin granule area and matrix metalloproteinase-13 (MMP-13) expression. These preventive effects on UVB-induced skin damage might be caused in part by inhibiting the degradation of extracellular matrixes in the corium, and by the proliferation of epidermal cells through the inhibition of increases in MMP-13 levels and reactive oxygen species induced by irradiation.
橄榄（Olea europaea L.）叶在欧洲和地中海地区一直被用于民间医学和草药茶。地中海气候的特点是高温和强烈的紫外线 B（UVB）辐射，导致皮肤老化，增加皱纹、色素沉着和皮肤厚度。本研究旨在研究橄榄叶提取物及其成分橄榄苦苷对 C57BL/6J 小鼠急性 UVB 照射引起的皮肤损伤的影响。提取物（300 或 1000mg/kg）和橄榄苦苷（25 或 85mg/kg）每天口服两次，连续 14 天。UVB 以 120mJ/cm2 的剂量每天照射一次，连续 5 天，然后每隔一天照射 9 天。两种处理均抑制了辐射引起的皮肤厚度增加。它们还抑制了 Ki-67 和 8-羟基-2'-脱氧鸟苷阳性细胞数量、黑色素颗粒面积和基质金属蛋白酶-13（MMP-13）表达的增加。这些对 UVB 诱导的皮肤损伤的预防作用可能部分是通过抑制真皮细胞外基质的降解，以及通过抑制照射引起的 MMP-13 水平和活性氧的增加来促进表皮细胞的增殖来实现的。
