从地不容毛状根培养物中生产千金藤素。

Dicentrine production from a hairy roots culture of Stephania suberosa.

机构信息

Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.

出版信息

Z Naturforsch C J Biosci. 2009 Sep-Oct;64(9-10):692-6. doi: 10.1515/znc-2009-9-1014.

DOI:10.1515/znc-2009-9-1014

PMID:19957438

Abstract

A hairy roots culture of Stephania suberosa was established using Agrobacterium rhizogenes ATCC15834. The production of dicentrine was found to be (8.92 +/- 0.07) mg/g dry wt on day 35 of culture. Effects of sucrose content, tyrosine, and medium strength on growth and dicentrine production of S. suberosa were investigated. 6% (w/v) sucrose was an optimum content for the growth and dicentrine accumulation in S. suberosa hairy roots. The utilization of a precursor from tyrosine feeding enhanced the dicentrine production. The medium with 1.0 mM of tyrosine had the highest effect on dicentrine accumulation in hairy roots at day 40 of culture [(14.73 +/- 0.47) mg/g dry wt]. In addition, 1/4 Murashige and Skoog medium was suitable for biomass and dicentrine production in hairy roots. This culture system has a potential to produce dicentrine from hairy roots of S. suberosa.

摘要

建立了一个利用发根农杆菌 ATCC15834 对金不换进行毛状根培养的体系。在培养的第 35 天，发现二千金碱的产量为（8.92±0.07）mg/g 干重。研究了蔗糖含量、酪氨酸和培养基强度对金不换毛状根生长和二千金碱产生的影响。6%（w/v）的蔗糖是金不换毛状根生长和二千金碱积累的最佳含量。利用酪氨酸前体的供给增强了二千金碱的产生。在培养的第 40 天，含 1.0 mM 酪氨酸的培养基对毛状根中二千金碱的积累有最高的影响[（14.73±0.47）mg/g 干重]。此外，1/4 Murashige 和 Skoog 培养基适合于毛状根中的生物量和二千金碱的产生。该培养体系有望从金不换的毛状根中产生二千金碱。