Department of Microbiology, Institute of Biology, Pedagogical University of Cracow, Podbrzezie 3, 31-054 Kraków, Poland.
Z Naturforsch C J Biosci. 2009 Sep-Oct;64(9-10):743-6. doi: 10.1515/znc-2009-9-1021.
The aim of the research was to determine the influence of strong hypergravity on the Paramecium bursaria-Chlorella sp. symbiotic association, which is considered to be a model example of symbiosis between a heterotroph and an autotroph. The paramecia cells were exposed to 1073 x g, 4293 x g, and 9658 x g hypergravity for 15 min. Then they were incubated for 21 d on a standard lettuce medium. The experiments were conducted in parallel under constant white light and in the dark. The changes in the number of paramecia cells during incubation were determined. Measurements of the number of Chlorella sp. endosymbionts inside host cells were also made. The results showed that a 15-min exposure to hypergravity attenuates the subsequent growth of Paramecium bursaria in the dark, but it may stimulate the growth of paramecia under constant light. Moreover, it causes an increase in the number of algae inside the paramecia cells. Presumably, the influence of hypergravity on the studied symbiotic complex is connected with its effect on the endosymbiotic Chlorella sp. cells. This subject requires further research, focused on the influence of hypergravity on the physiology and growth of the Chlorella sp. endosymbionts living inside the Paramecium bursaria cells.
本研究旨在探讨强超重对 Paramecium bursaria-Chlorella sp.共生体的影响，该共生体被认为是异养生物和自养生物共生的典型范例。将草履虫细胞暴露于 1073 x g、4293 x g 和 9658 x g 的超重环境中 15 分钟。然后，它们在标准生菜培养基上孵育 21 天。实验在恒定的白光和黑暗下平行进行。在孵育过程中，通过测定草履虫细胞的数量来确定其变化。同时，也对宿主细胞内 Chlorella sp.共生体的数量进行了测量。结果表明，15 分钟的超重暴露会削弱黑暗中 Paramecium bursaria 的后续生长，但在恒定光照下可能会刺激草履虫的生长。此外，它还会导致草履虫细胞内藻类数量的增加。推测超重对所研究共生复合体的影响与其对内生 Chlorella sp.细胞的影响有关。这一主题需要进一步的研究，重点关注超重对内生 Chlorella sp.共生体在 Paramecium bursaria 细胞内的生理和生长的影响。
