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[通过靶向对比超声心动图对P-选择素进行分子成像评估小鼠心肌缺血-再灌注损伤]

[Evaluation of myocardial ischemia-reperfusion injury in mouse by molecular imaging of P-selectin with targeted contrast echocardiography].

作者信息

Ji Li-Jing, Yang Li, Yan Yi, Wu Jue-Fei, Li Jun-Hua, Hu Guang-Quan, Bin Jian-Ping

机构信息

Cardiovascular Division, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2009 Jun 23;89(24):1698-701.

PMID:19957530
Abstract

OBJECTIVE

To assess the feasibility of evaluating myocardial ischemia-reperfusion injury in mouse with targeted myocardial contrast echocardiography (MCE).

METHODS

Phospholipid microbubbles targeted to P-selectin (MBp) and control microbubbles (MBc) were created by conjugating monoclonal antibody against murine P-selectin or isotype control antibody with the lipid shell via "avidin-biotin" bridging. Ten mice with myocardial ischemia-reperfusion were injected intravenously of MBp and MBc in a random order with a 30 min interval. After 5 min of intravenous injection of microbubble, targeted MCE imaging was performed in all mice. And then the video intensity (VI) was determined.

RESULTS

A significant ultrasonic enhancement was observed in ischemic region of MBp-group. Increment in VI value of ischemic region in MBp-group was great and it amounted to (26.0 +/- 6.2) U. However, increment in VI value of ischemic region in MBc-group was minor and it was merely (9.1 +/- 0.9) U. Difference was evident in ischemic region between of two groups (P < 0.05). In both MBp-group and MBc-group, the VI value of ischemic region was significantly greater than that of non-ischemic region (6.5 +/- 1.0) U vs (6.4 +/- 0.8) U (P < 0.05). There was no obvious difference in the VI of non-ischemic region between two groups.

CONCLUSION

Molecular imaging of P-selectin with targeted contrast echocardiography can effectively evaluate myocardial ischemia-reperfusion injury.

摘要

目的

评估采用靶向心肌对比超声心动图（MCE）评价小鼠心肌缺血再灌注损伤的可行性。

方法

通过“抗生物素蛋白-生物素”桥接将抗小鼠P-选择素单克隆抗体或同型对照抗体与脂质壳偶联，制备靶向P-选择素的磷脂微泡（MBp）和对照微泡（MBc）。10只心肌缺血再灌注小鼠，以随机顺序、间隔30分钟静脉注射MBp和MBc。静脉注射微泡5分钟后，对所有小鼠进行靶向MCE成像，然后测定视频强度（VI）。

结果

MBp组缺血区域观察到明显的超声增强。MBp组缺血区域的VI值增加显著，达（26.0±6.2）U。而MBc组缺血区域的VI值增加较小，仅为（9.1±0.9）U。两组缺血区域差异明显（P<0.05）。MBp组和MBc组缺血区域的VI值均显著高于非缺血区域（6.5±1.0）U对（6.4±0.8）U（P<0.05）。两组非缺血区域的VI无明显差异。

结论

靶向对比超声心动图对P-选择素进行分子成像可有效评估心肌缺血再灌注损伤。

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