阿魏酸钠治疗及干预机制逆转链脲佐菌素诱导的糖尿病大鼠勃起功能障碍

[Sodium ferulate treatment and interventional mechanism reverse erectile dysfunction in streptozotocin-induced diabetic rats].

作者信息

Xu Xiao-Hong, Tan Fu-Qing, Zhao Tong-Feng, Hu Hua, Xiao Kun, Gu Wei

机构信息

Department of Endocrinology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2009 Jun 23;89(24):1711-3.

PMID:19957533

Abstract

OBJECTIVE

To investigate the effect and mechanism of sodium ferulate (SF) on reversing erectile dysfunction in diabetes mellitus (DM) rats.

METHODS

Forty-four male adult Sprague-Dawley rats were induced for diabetes by an intraperitoneal injection of streptozotocin. Then the successful models were randomly divided into DM + SF group and DM group (22 rats each respectively). The rats in DM +SF group were treated with sodium ferulate (100 mg x kg(-1) x d(-1)) through a daily gastric lavage. At Week 12, the erectile function of all rats was evaluated and serum samples were harvested. The SOD, CAT, NOS, MDA and NO levels in corpus cavernosum and serum were all measured. The pathologic change in penile cavernous body was observed microscopically.

RESULTS

The diabetic rat models were successfully established. The erectile function was much better in normal control group and DM + SF group than that in DM group. And the penile erection rates in three groups were 100%, 66% and 22% respectively. The activity levels of SOD, CAT and NOS were markedly decreased in DM group as compared to those in normal control group and DM + SF group (P < 0.01). The NO content was approximately equal in normal control group and DM + SF group (112 +/- 28) nmol/ml vs (137 +/- 25) nmol/ml. But both were much higher than that in DM group (56 +/- 10) nmol/ml, both P < 0.01. The MDA content was markedly increased in DM group as compared to those in normal control group and DM + SF group (both P < 0.01). Microscopically, muscle fibers in penile cavernous body arranged disorderly, muscular mantle damaged and desmoplasia scattered among muscle fibers in DM group.

CONCLUSION

Sodium ferulate may play interventional roles in reversing diabetic erectile dysfunction through metabolic regulation of free radicals, antagonism of oxidative insults and enhancement of NO production.

摘要

目的

探讨阿魏酸钠（SF）对糖尿病（DM）大鼠勃起功能障碍的改善作用及机制。

方法

44只成年雄性Sprague-Dawley大鼠腹腔注射链脲佐菌素诱导糖尿病。将造模成功的大鼠随机分为DM + SF组和DM组（每组各22只）。DM + SF组大鼠每日灌胃给予阿魏酸钠（100 mg·kg⁻¹·d⁻¹）。第12周时，评估所有大鼠的勃起功能并采集血清样本。检测阴茎海绵体和血清中SOD、CAT、NOS、MDA和NO水平。显微镜下观察阴茎海绵体的病理变化。

结果

成功建立糖尿病大鼠模型。正常对照组和DM + SF组的勃起功能明显优于DM组。三组阴茎勃起率分别为100%、66%和22%。与正常对照组和DM + SF组相比，DM组SOD、CAT和NOS的活性水平显著降低（P < 0.01）。正常对照组和DM + SF组的NO含量相近（分别为(112 ± 28) nmol/ml和(137 ± 25) nmol/ml），但均显著高于DM组（(56 ± 10) nmol/ml），差异均有统计学意义（P < 0.01）。与正常对照组和DM + SF组相比，DM组MDA含量显著升高（均P < 0.01）。显微镜下，DM组阴茎海绵体肌纤维排列紊乱，肌套受损，肌纤维间有散在的发育异常。