Yao Le-shen, Wang Yang-tian, Chen Yun, Dai Yu-tian
Department of Urology, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China.
Zhonghua Nan Ke Xue. 2009 Oct;15(10):915-9.
To investigate the mechanism of diabetic erectile dysfunction (ED) and find new methods for its treatment by detecting the changes in nitric oxide synthase (NOS) isoforms and erectile function of diabetic rats and observing the effects of insulin and alpha-lipoic acid (LA) on it.
Fifty male Sprague-Dawley rats were divided into Groups A (normal control, n=10), B (non-intervention diabetes mellitus, n=13), C (insulin intervention diabetes mellitus, n=12), and D (insulin + LA intervention, n=15). And the diabetic models were made by intraperitoneal injection of streptozocin (STZ). Eight weeks later, the erectile function of the rats was assessed following apomorphine injection and the contents of NOS isoforms in the erectile tissues measured by immunohistochemical staining.
All the rats of Group A showed a normal erectile function (100%). In comparison, those in Groups B, C and D exhibited a significantly decreased rate, 28.6% in Group B, 62.5% in Group C and 80.9% in Group D. The numbers of positive nNOS fibers and eNOS in the penile tissues per visual field were 86.7 and 9.6 in Group A, but only 36.5 and 3.3 in Group B, 52.7 and 5.7 in Group C, and 71.4 and 7.4 in Group D (P < 0.05). However, the expression of iNOS was significantly lower in Group A (6.9) than in Groups B (43.6), C (36.2) and D (19.3) (P < 0.05). Compared with Groups B and C, the erectile function and the expressions of nNOS and eNOS were markedly increased, while the expression of iNOS significantly decreased in Group D (P < 0.05).
Diabetes mellitus severely affects penile erectile function and the expressions of NOS isoforms in the cavernous tissues, for which hyperglycemia is mainly responsible. LA is proved obviously efficacious for diabetic ED, which might be related to its antioxidant effect.
通过检测糖尿病大鼠一氧化氮合酶（NOS）亚型变化及勃起功能，观察胰岛素和α-硫辛酸（LA）对其的影响，探讨糖尿病性勃起功能障碍（ED）的发病机制并寻找新的治疗方法。
将50只雄性Sprague-Dawley大鼠分为A组（正常对照组，n = 10）、B组（糖尿病未干预组，n = 13）、C组（胰岛素干预糖尿病组，n = 12）和D组（胰岛素+LA干预组，n = 15）。通过腹腔注射链脲佐菌素（STZ）制备糖尿病模型。8周后，注射阿扑吗啡后评估大鼠勃起功能，采用免疫组织化学染色法检测勃起组织中NOS亚型含量。
A组所有大鼠勃起功能均正常（100%）。相比之下，B组、C组和D组大鼠勃起功能明显下降，B组为28.6%，C组为62.5%，D组为80.9%。A组阴茎组织每视野阳性nNOS纤维和eNOS数量分别为86.7和9.6，而B组仅为36.5和3.3，C组为52.7和5.7，D组为71.4和7.4（P < 0.05）。然而，A组iNOS表达（6.9）明显低于B组（43.6）、C组（36.2）和D组（19.3）（P < 0.05）。与B组和C组相比，D组勃起功能及nNOS和eNOS表达明显增加，而iNOS表达明显降低（P < 0.05）。
糖尿病严重影响阴茎勃起功能及海绵体组织中NOS亚型表达，高血糖为主要原因。LA对糖尿病性ED有明显疗效，可能与其抗氧化作用有关。
