Liu Jing, Yang Yang, Gong Ju-Li, Zhang Zi-Ning, Zhang Min, Wang Ya-Nan, Jiang Yong-Jun, Qi Zhong-Tian, Pan Qi-Chao, Zhong Ping, Shang Hong
The Key Laboratory of AIDS Immunology of the Ministry of Health, the First Hospital, China Medical University, Shenyang, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Jul;30(7):663-7.
To better understand the prevalence and geographic distribution of genotypes/subtypes on HCV and the relationship between HCV genotypes/subtypes and HIV infection disease progression in the HIV-1/HCV co-infected individuals living in high HIV-1 prevalent areas in China.
186 plasma samples were collected from HIV-1 seropositive individuals infected through paid blood donors (PBD), injecting drug users(IDUs) or sexual contact, living in most severely affected provinces, Henan, Yunnan, Xinjiang, Jilin and Liaoning provinces. Samples with HCV viral load >1000 cop/ml were amplified by RT-nested PCR, sequenced and phylogenetically analyzed for genotyping/subtyping of HCV. HIV-1, HCV viral loads and CD4+ T lymphocytes were measured for all subjects.
(1) HCV were identified as 1a (1.7%), 1b (39.9%), 2a (17.9%), 3a (10.4%), 3b (15.6%), 6a (1.2%), 6n (6.4%), and a newly unclassified subtype (7.5%). HCV 2a and 1b subtypes predominated in PBD in Henan, 3a and 3b in IDUs in Xinjiang and Yunnan, and 6 genotype/subtypes in IDU in Yunnan. (2) There were no significant differences in CD4+ T cell counts among the different HCV subtypes. (3) The viral load of HCV RNA in 1b subtype was higher than that of non-1b subtype, however, no significant differences in HIV-1 viral loads and CD4+ T cell counts were found between 1b and non-lb subtype. Both HIV and HCV viral loads were lower in 2a than non-2a subtype.
The prevalence of HCV genotype/subtype in HIV-1/HCV co-infected individuals was associated with geographic areas and transmission routes. HCV subtypes had no direct correlation with HIV infection disease progression.
更好地了解中国艾滋病病毒1型（HIV-1）高流行地区HIV-1/丙型肝炎病毒（HCV）合并感染个体中HCV基因型/亚型的流行情况和地理分布，以及HCV基因型/亚型与HIV感染疾病进展之间的关系。
从河南、云南、新疆、吉林和辽宁等受影响最严重省份的HIV-1血清阳性个体中采集186份血浆样本，这些个体通过有偿供血者（PBD）、注射吸毒者（IDU）或性接触感染。对HCV病毒载量>1000拷贝/ml的样本进行逆转录巢式聚合酶链反应（RT-nested PCR）扩增、测序，并进行系统发育分析以对HCV进行基因分型/亚型分型。对所有受试者检测HIV-1、HCV病毒载量和CD4+T淋巴细胞。
（1）HCV被鉴定为1a（1.7%）、1b（39.9%）、2a（17.9%）、3a（10.4%）、3b（15.6%）、6a（1.2%）、6n（6.4%）以及一种新的未分类亚型（7.5%）。HCV 2a和1b亚型在河南的有偿供血者中占主导，3a和3b亚型在新疆和云南的注射吸毒者中占主导，6基因型/亚型在云南的注射吸毒者中占主导。（2）不同HCV亚型之间的CD4+T细胞计数无显著差异。（3）1b亚型的HCV RNA病毒载量高于非1b亚型，然而，1b亚型和非1b亚型之间在HIV-1病毒载量和CD4+T细胞计数方面未发现显著差异。2a亚型的HIV和HCV病毒载量均低于非2a亚型。
HIV-1/HCV合并感染个体中HCV基因型/亚型的流行与地理区域和传播途径有关。HCV亚型与HIV感染疾病进展无直接相关性。
