Meng Ling-Xin, Ding Zhao-Jun, Chen Xi-Ping, Fei Li-Sheng, Chi Yu-Hua, Zhao Zhi-Gang, Wang Xiao-Fang, Hao Xi-Shan
Oncological Department, Rizhao People's Hospital, Rizhao Cancer Therapy Center, Rizhao 276826, China.
Zhonghua Nei Ke Za Zhi. 2009 Jul;48(7):562-5.
To investigate nerve growth factor (beta-NGF) and its receptors expression in human pancreatic ductal adenocarcinoma.
Expression and distribution of beta-NGF, tyrosine kinase A (TrKA) and P75(NGFR) were detected in operation tissue specimens of pancreatic ductal adenocarcinoma with immunohistochemistry and real-time PCR. Relations of beta-NGF and its receptors with clinical pathological characters, especially nerve invasion were analyzed.
beta-NGF and TrKA expression are higher in pancreatic adenocarcinoma than normal pancreas, and the differences are significant (P < 0.01). beta-NGF and TrKA expression are associated with the differentiation grades (DG), lymphatic node metastasis, nerve invasion and surgical pathological stages. Poorer of DG and later stages, more expression of beta-NGF and TrKA. beta-NGF and TrKA expression have positive correlations. beta-NGF, TrKA and P75(NGFR) mRNA expression have significantly increased 3.84, 4.23 and 2.41 times than normal tissues by real-time PCR, respectively.
beta-NGF and TrKA might play potential roles in carcinogenesis for pancreatic cancer, have affinity with clinicopathological characters of pancreatic cancer. beta-NGF and TrKA may have mutual effect in signal transduction leading to perineural invasion of pancreatic carcinoma.
研究神经生长因子（β-NGF）及其受体在人胰腺导管腺癌中的表达情况。
采用免疫组织化学和实时荧光定量PCR检测胰腺导管腺癌手术组织标本中β-NGF、酪氨酸激酶A（TrKA）和P75（NGFR）的表达及分布。分析β-NGF及其受体与临床病理特征，尤其是神经侵犯的关系。
胰腺腺癌中β-NGF和TrKA的表达高于正常胰腺，差异有统计学意义（P<0.01）。β-NGF和TrKA的表达与分化程度（DG）、淋巴结转移、神经侵犯及手术病理分期有关。DG越差、分期越晚，β-NGF和TrKA的表达越高。β-NGF和TrKA的表达呈正相关。实时荧光定量PCR结果显示，β-NGF、TrKA和P75（NGFR）mRNA表达分别比正常组织显著增加3.84、4.23和2.41倍。
β-NGF和TrKA可能在胰腺癌的发生发展中起潜在作用，与胰腺癌的临床病理特征密切相关。β-NGF和TrKA可能在导致胰腺癌神经周围侵犯的信号转导中相互作用。
