Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Ege, Izmir, Turkey.
Curr Eye Res. 2009 Nov;34(11):996-1003. doi: 10.3109/02713680903261405.
To determine the in vivo efficacy of cyclosporine A-loaded solid lipid nanoparticles (SLNs) in rabbit eyes.
SLNs were prepared and administered to the cul-de-sac of rabbits, and the drug amount in aqueous humor was detected by high performance liquid chromatography (HPLC). The irritation was evaluated by modified Draize testing.
The particle size of SLNs was detected as 225.9 +/- 5.5 nm with a negative surface charge. Aqueous humor drug levels reached 50.53 ng/mL, and there was no serious irritation in rabbit eyes.
Topical ophthalmic efficacy of cyclosporine A was enhanced via administration of SLNs.
确定环孢素 A 载药固体脂质纳米粒（SLNs）在兔眼内的体内疗效。
将 SLNs 制备并滴入兔眼穹窿部，通过高效液相色谱法（HPLC）检测房水中的药物含量。采用改良 Draize 试验评估刺激性。
SLNs 的粒径检测结果为 225.9±5.5nm，表面带负电荷。房水中的药物水平达到 50.53ng/mL，兔眼未见严重刺激。
通过 SLNs 给药，增强了环孢素 A 的眼部局部疗效。
