Sun Xicai, Wang Dehui, Yu Huapeng, Hu Li
Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
Acta Otolaryngol. 2010 May;130(5):607-13. doi: 10.3109/00016480903352975.
Collagen deposition was increased in regenerative rabbit maxillary mucosa, and the elevated collagen deposition during wound healing was associated with increased interleukin (IL)-6 and IL-8, and decreased IL-10.
To observe the natural progression of wound healing in rabbit maxillary sinus mucosa and to detect the expression of relevant cytokines.
A rabbit wound-healing model was established in the maxillary sinus. At different times after injury, the sizes of maxillary ostia were recorded, and histological changes in the sinus mucosa were examined by means of hematoxylin and eosin (H&E) and Masson trichrome staining. Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentrations of IL-6, IL-8, IL-10, and collagen types I and III.
A large amount of immature spindled collagen deposition was observed in the basal lamina and lamina propria of regenerative mucosa. IL-6 was increased significantly in regenerative mucosa after injury, especially at 14 days post wounding when compared with normal mucosa. IL-8 was also increased significantly in regenerative mucosa; however, IL-10 was decreased significantly when compared with normal mucosa. IL-6 and IL-8 showed a significant correlation with the expression level of collagen type I.
再生兔上颌黏膜中胶原蛋白沉积增加，伤口愈合过程中胶原蛋白沉积增加与白细胞介素（IL）-6和IL-8升高以及IL-10降低有关。
观察兔上颌窦黏膜伤口愈合的自然进程并检测相关细胞因子的表达。
在上颌窦建立兔伤口愈合模型。在损伤后的不同时间，记录上颌窦口大小，并用苏木精-伊红（H&E）和Masson三色染色法检查窦黏膜的组织学变化。采用酶联免疫吸附测定（ELISA）检测IL-6、IL-8、IL-10以及I型和III型胶原蛋白的浓度。
在再生黏膜的基膜和固有层中观察到大量不成熟的梭形胶原蛋白沉积。损伤后再生黏膜中IL-6显著增加，尤其是在受伤后14天时与正常黏膜相比。再生黏膜中IL-8也显著增加；然而，与正常黏膜相比，IL-10显著降低。IL-6和IL-8与I型胶原蛋白的表达水平呈显著相关性。
