Department of Oral and Maxillofacial Surgery, University of Kiel, Kiel, Germany.
Clin Oral Implants Res. 2010 Mar;21(3):262-7. doi: 10.1111/j.1600-0501.2009.01818.x. Epub 2009 Dec 4.
Hydroxyapatite (HA) and tricalcium phosphate (TCP) are two very common ceramic materials for bone replacement. A recently developed material for bone replacement is CeraBall, which is a mixed HA-TCP scaffold available as porous spherical scaffolds of diameter 4 and 6 mm. Before their use as bone replacement materials in vivo, in vitro testing of these scaffolds is necessary. The goal of this study was to characterise 4 and 6 mm CeraBall scaffolds in vitro with a view to their future use as bone replacement materials.
The proliferation of human mesenchymal stromal cells (hMSCs) seeded on CeraBall scaffolds was evaluated quantitatively using the WST [Water soluble tetrazolium ((4-[3-(4- Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1, 3-benzene disulfonate)] test and qualitatively by scanning electron microscopy (SEM). In addition, the standard MTT [(3-(4, 5-Dimenthylthiazol-2-Y1)-2, 5-Diphenyltetrazolium bromide)] biocompatibility test and cell vitality staining were performed using hMSCs. CeraBall scaffolds were also tested for their mechanical properties.
SEM and WST test results showed that hMSCs proliferated on CeraBall scaffolds over the course of 9 days. Proliferation was similar to that seen on tissue culture polystyrene (control). Cells showed a well-spread morphology and formed 'sheets' on the surface of scaffolds. Invasion of pores was observed. Good biocompatibility was demonstrated by MTT test results and cell vitality staining. Scaffolds of both 4 and 6 mm were able to withstand compressive loads of 5 N.
CeraBall scaffolds show good biocompatibility in vitro for hMSCs. This opens the way for in vivo applications.
羟基磷灰石（HA）和磷酸三钙（TCP）是两种非常常见的用于骨替代的陶瓷材料。最近开发的一种用于骨替代的材料是 CeraBall，它是一种混合 HA-TCP 支架，呈 4 和 6 毫米直径的多孔球形支架。在将这些支架作为骨替代材料体内使用之前，需要对其进行体外测试。本研究的目的是对 4 和 6 毫米 CeraBall 支架进行体外特性分析，以期将来将其用作骨替代材料。
通过 WST[水溶性四唑盐((4-[3-(4-碘苯基)-2-(4-硝基苯基)-2H-5-四唑]-1,3-苯二磺酸盐)]]试验对接种在 CeraBall 支架上的人间充质基质细胞（hMSCs）的增殖进行定量评估，并用扫描电子显微镜（SEM）进行定性评估。此外，还使用 hMSCs 对标准 MTT[(3-(4,5-二甲基噻唑-2-Y1)-2,5-二苯基四氮唑溴盐)]生物相容性试验和细胞活力染色进行了测试。还对 CeraBall 支架的机械性能进行了测试。
SEM 和 WST 试验结果表明，hMSCs 在 CeraBall 支架上增殖 9 天。增殖情况与组织培养聚苯乙烯（对照）相似。细胞形态良好，在支架表面形成“薄片”。观察到孔隙的侵入。MTT 试验结果和细胞活力染色表明具有良好的生物相容性。4 和 6 毫米的支架均能承受 5 N 的压缩载荷。
CeraBall 支架在体外对 hMSCs 具有良好的生物相容性。这为体内应用开辟了道路。
