Gao Ying, Zhang Bo
Department of Pathology, Health Science Center of Peking University, Beijing, 100191, P. R. China.
Ai Zheng. 2009 Dec;28(12):1277-82. doi: 10.5732/cjc.009.10162.
Telomerase transcriptional elements-interacting factor (TEIF) gene found recently by our research group is a transcription factor of a kind of human telomerase reverse transcriptase (hTERT) gene, and expresses in many kinds of tumor tissues. This study was to evaluate the expression of TEIF protein in human colorectal tumors and to explore its correlation with centrosome abnormality.
The expression of TEIF in 10 specimens of normal intestinal mucosa tissue, 30 specimens of colorectal cancer, and 54 specimens of colorectal adenoma was detected by immunohistochemistry. The expression of gamma-tubulin was detected by immunofluorescence.
Immunohistochemistry results showed that the differences of TEIF protein expression between the normal group and each tumor groups were statistically significant (P<0.01), and the difference of TEIF protein expression between the malignant tumor group and the benign group was not significant (P>0.05). TEIF strong positive rate (> or =++) was significantly higher in the carcinoma group than in the adenoma group or normal group (all P<0.001); the differences of TEIF strong positive rate between Grade I adenoma and Grade II or III adenoma were statistically significant (P<0.05), and the difference of TEIF strong positive rate between Grade II adenoma and Grade III adenoma was not statistically significant (P>0.05). Immunofluorescence results showed that centrosome amplification-positive rate was significantly higher in the colorectal cancer group than in the normal group or the adenoma group (both P<0.01); the difference of the centrosome amplification positive rate between Grade I adenoma and Grade III adenoma was statistically significant (P<0.05), and the differences of the centrosome amplification positive rate between Grade II adenoma and Grade I or III adenoma were statistically significant (P>0.05).
TEIF protein and centrosome amplification is commonly found in colorectal tumors. The expression level of TEIF is related to tumor histological grade and malignant degree. TEIF protein expression and centrosome amplification showed a high degree of positive correlation.
本研究团队最近发现的端粒酶转录元件相互作用因子（TEIF）基因是一种人类端粒酶逆转录酶（hTERT）基因的转录因子，在多种肿瘤组织中表达。本研究旨在评估TEIF蛋白在人大肠肿瘤中的表达，并探讨其与中心体异常的相关性。
采用免疫组织化学法检测10例正常肠黏膜组织、30例大肠癌组织及54例大肠腺瘤组织中TEIF的表达。采用免疫荧光法检测γ-微管蛋白的表达。
免疫组织化学结果显示，正常组与各肿瘤组之间TEIF蛋白表达差异有统计学意义（P<0.01），恶性肿瘤组与良性肿瘤组之间TEIF蛋白表达差异无统计学意义（P>0.05）。癌组织组TEIF强阳性率（≥++）显著高于腺瘤组和正常组（均P<0.001）；Ⅰ级腺瘤与Ⅱ级或Ⅲ级腺瘤之间TEIF强阳性率差异有统计学意义（P<0.05），Ⅱ级腺瘤与Ⅲ级腺瘤之间TEIF强阳性率差异无统计学意义（P>0.05）。免疫荧光结果显示，大肠癌组中心体扩增阳性率显著高于正常组和腺瘤组（均P<0.01）；Ⅰ级腺瘤与Ⅲ级腺瘤之间中心体扩增阳性率差异有统计学意义（P<0.05），Ⅱ级腺瘤与Ⅰ级或Ⅲ级腺瘤之间中心体扩增阳性率差异有统计学意义（P>0.05）。
TEIF蛋白与中心体扩增在大肠肿瘤中普遍存在。TEIF的表达水平与肿瘤组织学分级及恶性程度相关。TEIF蛋白表达与中心体扩增呈高度正相关。
