Obiefuna P C
Department of Physiology, College of Medical Sciences, University of Calabar, Nigeria.
J Trop Med Hyg. 1991 Feb;94(1):42-4.
Rouleaux formation of washed red blood cells (r.b.c.s) from subjects with sickle cell trait (HbAS) was measured by the rapid coagulation method. The r.b.c.s were suspended in the donor's native plasma or in 1.3% dextran in phosphate-buffered Ringer solution at pHs of 6.5, 7.4 and 8.2. HbAS cells showed more rouleaux formation than HbAA (normal) cells in plasma (P less than 0.005). Plasma fibrinogen concentration of HbAS and HbAA subjects did not differ significantly (mean 2.9, s.d. 0.7 g l-1 for both groups). In the dextran solution, HbAS cells were more aggregable at pH 6.5 but less at pH 8.2 than HbAA cells. There was no significant difference in dextran-induced aggregation between the two groups of cells at pH 7.4. The results suggest that HbAS subjects may be more prone to intravascular sludging and stasis and that this risk may be enhanced by reduction in pH of blood.
采用快速凝固法测定了镰状细胞性状（HbAS）受试者洗涤红细胞（r.b.c.s）的缗钱状形成。将红细胞悬浮于供体的天然血浆中，或悬浮于pH值为6.5、7.4和8.2的磷酸盐缓冲林格溶液中的1.3%右旋糖酐中。在血浆中，HbAS细胞比HbAA（正常）细胞表现出更多的缗钱状形成（P<0.005）。HbAS和HbAA受试者的血浆纤维蛋白原浓度无显著差异（两组均值均为2.9，标准差均为0.7 g l-1）。在右旋糖酐溶液中，pH值为6.5时HbAS细胞比HbAA细胞更易聚集，但在pH值为8.2时则相反。两组细胞在pH值为7.4时，右旋糖酐诱导的聚集无显著差异。结果表明，HbAS受试者可能更容易发生血管内淤滞和停滞，并且这种风险可能会因血液pH值降低而增加。
