KANG Zheng-Wu, JUNG Ji-Hun, KIM Sung Hun, LEE Bok Kwon, LEE Duk Young, KIM Young Jo, LEE Ji Youn, WON Ho-Keun, KIM Eun Hee, HAHN Tae-Wook
School of Veterinary Medicine, Kangwon National University, South Korea.
J Vet Med Sci. 2009 Nov;71(11):1433-8. doi: 10.1292/jvms.001433.
Salmonella Enteritidis is the most common cause of salmonellosis in humans in South Korea. It has been recognized that the principal source of human infection with S. Enteritidis is chickens and their products such as meat and eggs. A total of 173 S. Enteritidis isolates from humans (65 isolates) and chickens or their products (108 isolates) were analyzed by antibiotic susceptibility assay, phage typing, and pulsed-field gel electrophoresis (PFGE). Drug resistance was found to streptomycin (32.3%), ampicillin (30.6%), nalidixic acid (30.1%), ticarcillin (30.1%), and tetracycline (28.3%). More than 70% of the isolates were found to be resistant to one or more antibiotics tested. The most frequent patterns of resistant isolates were resistance to nalidixic acid only (28.3%) and resistance to two antibiotics (four combinations; 20.2%). The most predominant phage type (PT) was PT1 (27.2%) followed by PT21 (20.8%) and PT4 (8.7%) in chicken and human isolates. Nineteen different PFGE patterns were found among the 173 isolates, and A1 was the most common PFGE pattern, followed by A6 (17.3%). Most S. Enteritis isolates (except two isolates with patterns B and C) showed similar PFGE patterns that differed by only a few bands. These results show that 2 or 3 subtypes of S. Enteritidis are shared to a large extent by humans and chickens. This implies the possibility of the spread of chicken S. Enteritidis to humans.
肠炎沙门氏菌是韩国人类沙门氏菌病最常见的病因。人们已经认识到，人类感染肠炎沙门氏菌的主要来源是鸡及其产品，如肉类和蛋类。通过抗生素敏感性试验、噬菌体分型和脉冲场凝胶电泳（PFGE）对总共173株来自人类（65株）以及鸡或其产品（108株）的肠炎沙门氏菌分离株进行了分析。发现对链霉素（32.3%）、氨苄青霉素（30.6%）、萘啶酸（30.1%）、替卡西林（30.1%）和四环素（28.3%）耐药。超过70%的分离株对一种或多种测试抗生素耐药。耐药分离株最常见的模式是仅对萘啶酸耐药（28.3%）和对两种抗生素耐药（四种组合；20.2%）。在鸡和人类分离株中，最主要的噬菌体类型（PT）是PT1（27.2%），其次是PT21（20.8%）和PT4（8.7%）。在173株分离株中发现了19种不同的PFGE模式，A1是最常见的PFGE模式，其次是A6（17.3%）。大多数肠炎沙门氏菌分离株（除了两株模式为B和C的分离株）显示出相似的PFGE模式，仅相差几条带。这些结果表明，肠炎沙门氏菌的2种或3种亚型在很大程度上为人类和鸡所共有。这意味着鸡源肠炎沙门氏菌有可能传播给人类。
