碳酸酐酶同工酶CA-II和CA-VI在犬类下呼吸道和肺中的免疫组织化学定位及基因表达

Immunohistochemical localization and gene expression of carbonic anhydrase isoenzymes CA-II and CA-VI in canine lower airways and lung.

作者信息

SUGIURA Yosuke, OISHI Motoharu, AMASAKI Tomoko, SOETA Satoshi, ICHIHARA Nobutsune, NISHITA Toshiho, MURAKAMI Masaru, AMASAKI Hajime, ASARI Masao

机构信息

Department of Anatomy, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.

出版信息

J Vet Med Sci. 2009 Nov;71(11):1525-8. doi: 10.1292/jvms.001525.

DOI:10.1292/jvms.001525

PMID:19959906

Abstract

The immunohistolocalization and gene expression of carbonic anhydrase (CA) isoenzymes CA-II and CA-VI in the canine lower airways and lung were examined using specific canine CA-II and CA-VI antisera and the RT-PCR method. Laryngeal, tracheal and bronchial epithelia, serous acinar and bronchiolar secretory cells and pulmonary great alveolar cells showed immunopositive reactions to anti-CA-II and anti-CA-VI antisera. However, all mucous cells showed immunonegative reactions. The physiological roles of CA-II and CA-VI in the lower airways and lung may involve the maintenance of pH balance and the protection of mucosal surfaces against the acidic milieu.

摘要

利用特异性犬CA-II和CA-VI抗血清及逆转录-聚合酶链反应（RT-PCR）方法，检测了碳酸酐酶（CA）同工酶CA-II和CA-VI在犬下呼吸道和肺中的免疫定位及基因表达。喉、气管和支气管上皮、浆液性腺泡和细支气管分泌细胞以及肺大肺泡细胞对抗CA-II和抗CA-VI抗血清呈免疫阳性反应。然而，所有黏液细胞均呈免疫阴性反应。CA-II和CA-VI在下呼吸道和肺中的生理作用可能涉及维持pH平衡以及保护黏膜表面免受酸性环境的影响。