利用激光显微切割系统对犬唾液腺纹状管中分泌性碳酸酐酶同工酶的基因表达研究

Gene expression of secretory carbonic anhydrase isozymes in striated ducts of canine salivary glands using laser microdissection system.

作者信息

Ichihara N, Tsukamoto A, Kasuya T, Shibata S, Nishita T, Murakami M, Amasaki H, Asari M

机构信息

Laboratory of Anatomy I, Azabu University School of Veterinary Medicine, Fuchinobe 1-17-71, Sagamihara, Kanagawa 229-8501, Japan.

出版信息

Anat Histol Embryol. 2007 Oct;36(5):357-60. doi: 10.1111/j.1439-0264.2007.00774.x.

DOI:10.1111/j.1439-0264.2007.00774.x

PMID:17845225

Abstract

To clarify whether striated duct cells in canine salivary glands synthesize secretory carbonic anhydrase (CA-VI), as is the case with serous acinar cells, the present study utilized laser microdissection to harvest striated duct cells from canine parotid and submandibular glands, and total RNA extracted from these cells was then amplified by reverse transcription-polymerase chain reaction to assess CA-VI gene expression. The results confirmed the local expression of CA-VI mRNA in striated duct cells. This clarified that, in canine salivary glands, CA-VI is synthesized in not only serous acinar cells, but also striated duct cells.

摘要

为了明确犬唾液腺的纹状管细胞是否像浆液性腺泡细胞那样合成分泌性碳酸酐酶（CA-VI），本研究利用激光显微切割技术从犬腮腺和下颌下腺采集纹状管细胞，然后从这些细胞中提取总RNA，通过逆转录-聚合酶链反应进行扩增，以评估CA-VI基因的表达。结果证实了CA-VI mRNA在纹状管细胞中的局部表达。这表明，在犬唾液腺中，不仅浆液性腺泡细胞能合成CA-VI，纹状管细胞也能合成。