OZAWA Manao, YAMAMOTO Kinya, KOJIMA Akemi, TAKAGI Masami, TAKAHASHI Toshio
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo, Japan.
J Vet Med Sci. 2009 Nov;71(11):1537-40. doi: 10.1292/jvms.001537.
In order to investigate the origin of tetracycline resistance in Erysipelothrix rhusiopathiae, conjugative transpositions of Tn916 were tested. The frequency of transfer between strains of E. rhusiopathiae was about 10-fold higher than that between Enterococcus faecalis and E. rhusiopathiae. In addition, detection of a Tn916-like transposon was performed by PCR assay and DNA sequencing in E. rhusiopathiae field isolates. Of 49 tetracycline-resistant isolates, 38 (77.6 %) carried a Tn916-like transposon, while 11 (22.4 %) carried tet(M) only. These results suggested that Tn916-like transposon may be widely present in the E. rhsuiopathiae field isolates resistant to tetracycline.
为了研究猪丹毒丝菌中四环素抗性的起源，对Tn916的接合转座进行了检测。猪丹毒丝菌菌株间的转移频率比粪肠球菌和猪丹毒丝菌之间的转移频率高约10倍。此外，通过PCR检测和DNA测序对猪丹毒丝菌田间分离株进行了Tn916样转座子的检测。在49株四环素抗性分离株中，38株（77.6%）携带Tn916样转座子，而11株（22.4%）仅携带tet(M)。这些结果表明，Tn916样转座子可能广泛存在于对四环素耐药的猪丹毒丝菌田间分离株中。
