Department of Microbiology and Molecular Biology, Brigham Young University, WIDB 893, Provo, UT 84602, USA.
J Med Entomol. 2009 Nov;46(6):1532-6. doi: 10.1603/033.046.0642.
The microbial flora associated with fleas may affect their ability to transmit specific pathogens, including Yersinia pestis, and also could be used to develop paratransgenesis-based approaches to interfere with transmission. To begin addressing this hypothesis, the microbial flora associated with the relatively efficient Y. pestis vectors Xenopsylla cheopis (Rothschild) (Siphonaptera: Pulicidae) and Oropsyllamontana (Baker) (Siphonaptera: Ceratophyllidae), and the inefficient vector Ctenocephalides felis felis (Bouché) (Siphonaptera: Pulicidae) were investigated using polymerase chain reaction amplification of 16S rDNA genes. DNA sequencing revealed that these species harbor distinct communities of microbial flora and suggest that Acinetobacter sp. might be used in developing anti-transmission strategies.
与跳蚤相关的微生物菌群可能会影响其传播特定病原体的能力，包括鼠疫耶尔森菌，也可以用来开发基于共生菌的方法来干扰传播。为了开始验证这一假设，我们使用聚合酶链反应扩增 16S rDNA 基因的方法来研究相对高效的鼠疫耶尔森菌载体印鼠客蚤（Xenopsylla cheopis）（蚤目：蚤科）和蒙塔那纤蚤（Oropsylla montana）（蚤目：角叶蚤科），以及低效载体猫栉首蚤（Ctenocephalides felis felis）（蚤目：蚤科）相关的微生物菌群。DNA 测序结果表明，这些物种携带有独特的微生物菌群，并表明不动杆菌属（Acinetobacter sp.）可能用于开发抗传播策略。
