[系统性红斑狼疮患者中医证型与外周血单个核细胞中IL-10、IL-18及Fas mRNA表达的关系]

[Relationship between Chinese medicine syndrome type and mRNA expressions of IL-10, IL-18 and Fas in peripheral blood mononuclear cells in patients with systemic lupus erythematosus].

作者信息

Zhang Jun-Ling, Gong Ze-Kun

机构信息

School of Chemical Engingeering and Technology of Tianjin University, Tianjin.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Sep;29(9):783-6.

PMID:19960972

Abstract

OBJECTIVE

To explore the mRNA expressions of IL-10, IL-18 and Fas in peripheral blood mononuclear cells (PBMCs) in systemic lupus erythematosus (SLE) patients of various Chinese medicine syndrome types in order to seek the objective indices for syndrome differentiation of SLE.

METHODS

SLE patients were sorted into various syndrome types by Chinese medicine syndrome differentiation. In them, patients with the 4 often encountered types were selected, i.e. the qi-xue excessive heat type (Group A, 12 patients), the Pi-Shen yang-deficiency type (Group B, 19 patients), the qi-yin deficiency with blood stasis type (Group C, 23 patients), and the qi stagnation and blood stasis with gan stagnation type (Group D, 28 patients). The mRNA expressions of IL-10, IL-18 and Fas in PBMCs were detected by SYBR Green I real time PCR.

RESULTS

The mRNA expressions of IL-10, IL-18 and Fas in PBMC of all SLE patients (0.40 +/- 0.53, 1.41 +/- 1.93 and 0.33 +/- 0.70) were significantly higher than those in the normal control group (0.07 +/- 0.11, 0.24 +/- 0.25, 0.08 +/- 0.21, P<0.01, P<0.05), their levels in different syndrome types decreased in order of type A-->B-->C-->D. The levels of the three indices in patients of type A and B were obviously higher than those in patients of type C and D (P<0.01), while no significant difference was found between type A and B (P>0.05), and between type C and D (P>0.05).

CONCLUSION

The mRNA expressions of IL-10, IL-18 and Fas in PBMCs could be taken as the objective indices for reference in syndrome differentiation of SLE.

摘要

目的

探讨系统性红斑狼疮（SLE）不同中医证型患者外周血单个核细胞（PBMCs）中白细胞介素10（IL-10）、白细胞介素18（IL-18）和Fas的mRNA表达情况，以寻求SLE辨证的客观指标。

方法

采用中医辨证对SLE患者进行证型分类。从中选取4种常见证型的患者，即气血实热型（A组，12例）、脾肾阳虚型（B组，19例）、气阴两虚兼血瘀型（C组，23例）和气滞血瘀兼肝郁型（D组，28例）。采用SYBR Green I实时荧光定量PCR法检测PBMCs中IL-10、IL-18和Fas的mRNA表达。

结果

所有SLE患者PBMCs中IL-10、IL-18和Fas的mRNA表达水平（0.40±0.53、1.41±1.93和0.33±0.70）均显著高于正常对照组（0.07±0.11、0.24±0.25、0.08±0.21，P<0.01，P<0.05），不同证型患者上述指标水平按A组→B组→C组→D组顺序递减。A组和B组患者上述3项指标水平明显高于C组和D组患者（P<0.01），而A组与B组之间（P>0.05）、C组与D组之间（P>0.05）差异无统计学意义。