Matsuoka Masanori, Gonzalez Alberto Vargas, Estrada Iris, Carreño-Martinez Cristina, Fafutis-Morris Mary
Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1, Aobacho, Higashimurayama-shi, Tokyo, 189-0002, Japan.
Lepr Rev. 2009 Sep;80(3):322-6.
To classify Mycobacterium leprae isolates from multiple areas in Mexico based on variable number of tandem repeats of 6 base within the rpoT gene and three single nucleotide polymorphism (SNP), and to analyse their geographic distribution in the context of the origin of leprosy in Mexico.
Analysis for rpoT genotyping of 64 samples collected in the west and southwestern areas revealed that 46 isolates were of the 4 copy type and 18 isolates were of the 3 copy type. All samples from the eastern coastal area (n = 24) and from the Yucatan peninsula (n = 12) were of the 3 copy type. Six isolates from the west and southwestern area were SNP-type 1, 13 isolates were SNP-type 2 and 45 isolates were SNP-type 3. Nineteen of 24 isolates from the eastern coastal area were SNP-type 3 and one was SNP-type 4. Seven isolates from the Yucatan peninsula were SNP-type 3 and one was SNP-type 4.
The difference of the proportion of each genotype between the western areas and the eastern areas indicated the expansion of leprosy through different paths in Mexico.
基于rpoT基因内6碱基串联重复序列的可变数目以及三个单核苷酸多态性（SNP）对来自墨西哥多个地区的麻风分枝杆菌分离株进行分类，并结合墨西哥麻风病的起源背景分析其地理分布。
对在西部和西南部地区收集的64份样本进行rpoT基因分型分析，结果显示46株分离株为4拷贝型，18株分离株为3拷贝型。来自东部沿海地区（n = 24）和尤卡坦半岛（n = 12）的所有样本均为3拷贝型。来自西部和西南部地区的6株分离株为SNP-1型，13株为SNP-2型，45株为SNP-3型。来自东部沿海地区的24株分离株中有19株为SNP-3型，1株为SNP-4型。来自尤卡坦半岛的7株分离株为SNP-3型，1株为SNP-4型。
西部地区和东部地区各基因型比例的差异表明麻风病在墨西哥通过不同途径传播。
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