Cardona-Castro Nora, Beltrán-Alzate Juan Camilo, Romero-Montoya Irma Marcela, Meléndez Esperanza, Torres Fernando, Sakamuri Rama Murthy, Li Wei, Vissa Varalakshmi
Instituto Colombiano de Medicina Tropical (ICMT)--Universidad CES, Cra 43A No. 52 Sur 99. Sabaneta, Antioquia, Colombia.
Lepr Rev. 2009 Sep;80(3):316-21.
To evaluate and establish genomic strain typing markers suitable for the identification of transmission patterns of leprosy in different regions of Colombia.
Patients from Agua de Dios, Barranquilla and Cartagena cities and neighbouring towns were enrolled during 2006-2007. Slit skin smears or biopsies were obtained from newly detected untreated patients, and those undergoing multidrug therapy. DNA was extracted from the clinical samples and tested using 15 different short tandem repeat and three SNP polymorphic markers.
Differences or similarities between strain types from the northeast (n = 20) and central regions of Colombia (n = 18) were noted. The alleles at two loci, 27-5 and 12-5 were different in the M. leprae in the two regions. The other microsatellite loci may be useful for further intra-population differentiation. There was strong association of 27-5 and 12-5 alleles with the SNP types. The 4-5 combination of alleles was associated with SNP type 3, while the 5-4 combination was mostly associated with SNP type 1, 2 or 4. The SNP type 4 m. leprae isolates were seen in patients in the northeast, but not in the central part.
评估并建立适用于鉴定哥伦比亚不同地区麻风病传播模式的基因组菌株分型标记。
2006年至2007年期间，招募了来自阿瓜德迪奥斯、巴兰基亚和卡塔赫纳市及周边城镇的患者。从新发现的未经治疗的患者以及接受多药治疗的患者身上获取 slit 皮肤涂片或活检样本。从临床样本中提取DNA，并使用15种不同的短串联重复序列和三种单核苷酸多态性标记进行检测。
注意到来自哥伦比亚东北部（n = 20）和中部地区（n = 18）的菌株类型之间存在差异或相似性。两个区域的麻风分枝杆菌中，两个位点27 - 5和12 - 5的等位基因不同。其他微卫星位点可能有助于进一步进行群体内分化。27 - 5和12 - 5等位基因与单核苷酸多态性类型有很强的关联。等位基因的4 - 5组合与单核苷酸多态性类型3相关，而5 - 4组合主要与单核苷酸多态性类型1、2或4相关。单核苷酸多态性类型4的麻风分枝杆菌分离株在东北部患者中可见，但在中部地区未发现。
