Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, University of Toronto, Ontario, Canada.
J Neurosurg. 2011 Feb;114(2):441-5. doi: 10.3171/2009.10.JNS08259. Epub 2009 Dec 4.
The authors investigated the feasibility of using fat allografts (chemically treated to reduce the host immune response) for neurosurgical applications.
Subcutaneous fat specimens collected from New Zealand White rabbits were treated with DNAse I and sodium deoxycholate to reduce immunogenicity before subcutaneous, midscapular implantation in immunocompetent recipient rabbits. Allograft incorporation and the host-allograft response were examined at 1, 6, and 11 weeks by histopathological analysis. Control specimens of autograft and untreated fat allograft implants were examined for comparison.
The host immune response was markedly reduced in the region around the chemically treated fat allografts when compared with untreated allografts, and was similar to the tolerant host response to autografts.
Based on their results, the authors suggest that fat allografts processed for reduced immunogenicity may be a convenient, viable alternative for neurosurgical applications.
作者研究了使用脂肪同种异体移植物（经化学处理以降低宿主免疫反应）进行神经外科应用的可行性。
从新西兰白兔中收集皮下脂肪标本，用 DNAse I 和脱氧胆酸钠处理以降低免疫原性，然后将其皮下、肩胛间植入免疫功能正常的受体兔。通过组织病理学分析，在 1、6 和 11 周时检查同种异体移植物的合并情况和宿主-同种异体移植物反应。为了比较，还检查了自体移植物和未经处理的脂肪同种异体移植物植入物的对照标本。
与未经处理的同种异体移植物相比，经化学处理的脂肪同种异体移植物周围的宿主免疫反应明显降低，与对自体移植物的耐受宿主反应相似。
根据他们的结果，作者建议，经过降低免疫原性处理的脂肪同种异体移植物可能是神经外科应用的一种方便、可行的替代方法。
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