马脂肪和肝脏组织中的从头脂肪酸合成和 NADPH 生成。

De novo fatty acid synthesis and NADPH generation in equine adipose and liver tissue.

机构信息

Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24060, USA.

出版信息

Comp Biochem Physiol B Biochem Mol Biol. 2010 Mar;155(3):322-6. doi: 10.1016/j.cbpb.2009.11.019. Epub 2009 Dec 3.

DOI:10.1016/j.cbpb.2009.11.019

PMID:19962447

Abstract

The lipogenic capacities of equine liver and adipose tissue explants were investigated in vitro. Preference for glucose or acetate as the primary carbon source for de novo fatty acid synthesis was determined using (14)C labeled substrates. Additional aims included determining the relative contribution of NADPH generating pathways to reducing equivalent generation and comparing the lipogenic activity of two adipose depots, mesenteric and subcutaneous harvested from the crest region of the neck. Mesenteric adipose tissue had greater lipogenic activity than subcutaneous adipose tissue, and liver tissue showed minimal (14)C incorporation into fatty acids, indicating a low hepatic lipogenic capacity. Acetate was found to be the primary carbon source for fatty acid synthesis due to both the appearance of the (14)C label in the lipid fraction and the low activity of ATP-citrate lyase. Finally, the pentose phosphate and isocitrate dehydrogenase enzymes contributed to NADPH production in equine adipose tissue.

摘要

本研究采用离体培养的方法研究了马肝脏和脂肪组织的生脂能力。利用（14）C 标记的底物确定葡萄糖或乙酸盐作为从头合成脂肪酸的主要碳源的偏好性。其他目的还包括确定 NADPH 生成途径对还原当量生成的相对贡献，并比较来自颈部嵴区域的肠系膜和皮下两种脂肪组织的生脂活性。肠系膜脂肪组织的生脂活性大于皮下脂肪组织，而肝脏组织几乎没有（14）C 掺入脂肪酸，表明肝脏的生脂能力较低。由于脂质部分出现（14）C 标记以及 ATP-柠檬酸裂解酶活性较低，因此发现乙酸盐是脂肪酸合成的主要碳源。最后，磷酸戊糖和异柠檬酸脱氢酶酶在马脂肪组织中产生 NADPH。