Katsuki S, Arnold W P, Murad F
J Cyclic Nucleotide Res. 1977 Aug;3(4):239-47.
Three agents that activate guanylate cyclase, sodium nitroprusside, nitroglycerin and sodium axide, were examined for their effects on cyclic GMP and cyclic AMP accumulation and muscle motility with several tissues. All of these agents, except nitroglycerin with ventricle preparations, increased cyclic GMP levels and did not alter cyclic AMP in incubations of preparations of bovine tracheal smooth muscle, guinea pig tracheal chains, taenia cecum, atria and ventricle, and rat liver and cerebral cortex. Increases in cyclic GMP with these agents occurred with relaxation of smooth muscle preparations and without alteration in the contractility of atrial preparations. These observations support the hypothesis that cyclic GMP accumulation in smooth muscle may be related to relaxation rather than contraction as proposed previously. Relaxation with these agents is not associated with alterations in cyclic AMP levels. Increases in cyclic GMP levels in atrial preparations can also occur without changes in contractile force or rate of contraction.
研究了三种激活鸟苷酸环化酶的药物，硝普钠、硝酸甘油和氧化钠，观察它们对几种组织中环鸟苷酸（cGMP）和环腺苷酸（cAMP）积累以及肌肉运动的影响。除了硝酸甘油对心室标本外，所有这些药物在牛气管平滑肌、豚鼠气管链、盲肠带、心房和心室以及大鼠肝脏和大脑皮层标本的孵育中均能提高cGMP水平，而不改变cAMP水平。这些药物使cGMP增加的同时，平滑肌标本松弛，心房标本的收缩性未改变。这些观察结果支持了如下假说：平滑肌中cGMP的积累可能如先前提出的那样与舒张而非收缩有关。这些药物引起的舒张与cAMP水平的改变无关。心房标本中cGMP水平升高时，收缩力或收缩速率也可能不变。
