Klose A D, Beattie B J
Department of Radiology at Columbia University, New York, NY 10027, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:6327-30. doi: 10.1109/IEMBS.2009.5333170.
We present a three-dimensional (3D) bioluminescence image reconstruction method with MRI and CT co-registration for small animal molecular imaging. The multi-spectral light intensity distribution of an optical luciferase-luciferin reporter system is measured at the tissue surface of a small animal for the purpose of 3D image reconstruction. The reporter probe distribution inside tissue is calculated with a linear matrix inversion method and a light propagation model based on the simplified spherical harmonics equations. The animal's surface geometry and anatomy is determined from co-registered CT and MR images in order to locate the reconstructed source distribution relative to the animal's anatomy. We present in vivo bioluminescence reconstruction results that demonstrate the performance of our co-registration method.
我们提出了一种用于小动物分子成像的、具有MRI和CT配准功能的三维（3D）生物发光图像重建方法。为了进行3D图像重建，在小动物的组织表面测量了光学荧光素酶-荧光素报告系统的多光谱光强分布。利用线性矩阵反演方法和基于简化球谐方程的光传播模型计算组织内部的报告探针分布。通过配准的CT和MR图像确定动物的表面几何形状和解剖结构，以便相对于动物的解剖结构定位重建的源分布。我们展示了体内生物发光重建结果，证明了我们配准方法的性能。
