在固定有碳纳米管的微电极上培养的发育中神经元中电活动的早期发生。

Early onset of electrical activity in developing neurons cultured on carbon nanotube immobilized microelectrodes.

作者信息

Khraiche Massoud Louis, Jackson Nathan, Muthuswamy Jit

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:777-80. doi: 10.1109/IEMBS.2009.5333590.

DOI:10.1109/IEMBS.2009.5333590

PMID:19964241

Abstract

In this study, we test the hypothesis that increased surface roughness due to carbon nanotubes (CNTs) enhances neuronal adhesion and consequently electrical excitability of single neurons. Neurons are grown on CNT modified microelectrode arrays (MEAs). Multi-unit activity was seen as early as 4 days after seeding compared to 7 days in control cultures on microelectrodes without CNTs. The results overall, show earlier onset and higher level of electrical activity in neurons seeded on CNT modified MEAs compared to non-modified control MEAs. We conclude that CNTs on microelectrodes enhance electrical excitability of single neurons in culture.

摘要

在本研究中，我们验证了这样一个假设：碳纳米管（CNT）导致的表面粗糙度增加会增强神经元黏附，进而提高单个神经元的电兴奋性。神经元在经碳纳米管修饰的微电极阵列（MEA）上生长。与在无微碳纳米管修饰的微电极上培养7天相比，接种后仅4天就能观察到多单元活动。总体结果表明，与未修饰的对照微电极阵列相比，接种在经碳纳米管修饰的微电极阵列上的神经元电活动起始更早且水平更高。我们得出结论，微电极上的碳纳米管可提高培养中单个神经元的电兴奋性。