Soumetz Federico Caneva, Pastorino Laura, Ruggiero Carmelina
Department of Communication, Computer and System Sciences, University of Genova, Italy.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:2775-8. doi: 10.1109/IEMBS.2009.5333860.
Monoclonal antibodies were immobilized onto the surface of quartz crystals for the development of a piezoelectric biosensor by means of the Layer by Layer self assembly technique (LBL). The immobilization of immunoglobulins specific to the matrix metalloproteinase-1 (MMP-1) was investigated. To this purpose multilayered ultra-thin films composed by precursor layers of cationic poly(dimethyldiallylammonium) chloride and anionic poly(styrenesulfonate) followed by a monolayer of antibodies were assembled by LBL. A quartz crystal microbalance was used to monitor and optimize the assembly process and to test the immunological activity of the deposited antibody molecules. Atomic force microscopy was used to characterize the surface roughness of the multilayers before and after the deposition of the immunoglobulins. The obtained results confirmed the successful deposition of the proposed immunosensor and demonstrated its high potential for the measurement of analytes of clinical interest.
通过层层自组装技术（LBL）将单克隆抗体固定在石英晶体表面，以开发一种压电生物传感器。研究了针对基质金属蛋白酶-1（MMP-1）的免疫球蛋白的固定化。为此，通过LBL组装了由阳离子聚（二甲基二烯丙基氯化铵）和阴离子聚（苯乙烯磺酸盐）的前体层组成的多层超薄膜，随后是单层抗体。使用石英晶体微天平监测和优化组装过程，并测试沉积的抗体分子的免疫活性。原子力显微镜用于表征免疫球蛋白沉积前后多层膜的表面粗糙度。所得结果证实了所提出的免疫传感器的成功沉积，并证明了其在测量具有临床意义的分析物方面的高潜力。
