Li Xiang, Jin Qiaofeng, Chen Tan, Zhang Baoyue, Zheng Rongqin, Wang Zhanhui, Zheng Hairong
Paul Lauterbur Center for Biomedical imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:463-6. doi: 10.1109/IEMBS.2009.5334473.
Using ultrasonic contrast microbubbles as acoustic biomarkers and drug carrier vehicles by conjugating tumor specific antibody to microbubbles has shown great potential in ultrasonic tumor molecular imaging or drug-delivery and therapy. Microbubble probe targeting efficiency is one of the major challenges. In this study, we developed a novel method to evaluate the targeting capability and efficiency of microbubbles to cells, and more specifically, microbubbles binding LyP-1 (a cyclic nonapeptide acid peptide) target to cancer cell within a microfluidic system. The micro cell sieves within the microfludic channels could trap the tumor cells and enhance the microbubble's interaction with the cell. Assisted with the controllable fluid shear stress, the microbubble's targeting to the cell and the corresponding affinity efficiency could be quantitatively evaluated under a florescent microscope. The system provides a useful low-cost high efficient in vitro platform for studying microbubble-cell interaction for ultrasonic tumor molecular imaging or drug-delivery and therapy.
通过将肿瘤特异性抗体与微泡偶联，使用超声造影微泡作为声学生物标志物和药物载体，在超声肿瘤分子成像或药物递送及治疗方面已显示出巨大潜力。微泡探针的靶向效率是主要挑战之一。在本研究中，我们开发了一种新方法来评估微泡对细胞的靶向能力和效率，更具体地说，是在微流控系统中评估结合LyP-1（一种环状九肽酸肽）靶点的微泡与癌细胞的结合情况。微流控通道内的微细胞筛可以捕获肿瘤细胞并增强微泡与细胞的相互作用。在可控的流体剪切应力辅助下，可在荧光显微镜下对微泡与细胞的靶向作用及相应的亲和效率进行定量评估。该系统为研究用于超声肿瘤分子成像或药物递送及治疗的微泡-细胞相互作用提供了一个有用的低成本高效体外平台。
