Opoku J, Kalimi M, Agarwal M, Qureshi D
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Am J Physiol. 1991 Feb;260(2 Pt 1):E269-71. doi: 10.1152/ajpendo.1991.260.2.E269.
The effects of the mineralocorticoid antagonist mespirenone on the development and maintenance of aldosterone-induced hypertension in Sprague-Dawley rats has been studied. Uninephrectomized saline-drinking male Sprague-Dawley rats were injected with either 0.2 ml olive oil, 50 g aldosterone, 1 mg mespirenone, 50 g aldosterone plus 500 g mespirenone, or 50 g aldosterone plus 1 mg mespirenone, each dissolved in 0.2 ml olive oil. Administration of aldosterone alone significantly increased the systolic blood pressure (SBP) from a control value of 114 +/- 3.6 to 162 +/- 4 mmHg by the end of the 3-wk experimental period. Mespirenone given alone had no effect on SBP. However, mespirenone given in combination with aldosterone reversed the hypertension caused by aldosterone in a dose-dependent manner. Saline consumption and urinary output were slightly increased in aldosterone-treated rats compared with the other groups, but the body and organ weights were comparable in all groups. Microscopic examination of kidney and heart showed no abnormalities due to mespirenone. These results suggest that in vivo administration of mespirenone to Sprague-Dawley rats effectively prevents the aldosterone-induced hypertension.
研究了盐皮质激素拮抗剂美螺内酯对Sprague-Dawley大鼠醛固酮诱导的高血压发展和维持的影响。将单侧肾切除并饮用生理盐水的雄性Sprague-Dawley大鼠分别注射0.2 ml橄榄油、50 μg醛固酮、1 mg美螺内酯、50 μg醛固酮加500 μg美螺内酯或50 μg醛固酮加1 mg美螺内酯，每种均溶于0.2 ml橄榄油中。单独给予醛固酮在3周实验期结束时可使收缩压（SBP）从对照值114±3.6显著升高至162±4 mmHg。单独给予美螺内酯对SBP无影响。然而，美螺内酯与醛固酮联合使用可剂量依赖性地逆转醛固酮引起的高血压。与其他组相比，醛固酮处理的大鼠的盐水消耗量和尿量略有增加，但所有组的体重和器官重量相当。对肾脏和心脏的显微镜检查未发现美螺内酯导致的异常。这些结果表明，对Sprague-Dawley大鼠体内给予美螺内酯可有效预防醛固酮诱导的高血压。
