Institute of Molecular Systems Biology, Swiss Federal Institute of Technology, Zurich, Switzerland.
Nat Methods. 2010 Jan;7(1):43-6. doi: 10.1038/nmeth.1408. Epub 2009 Dec 6.
Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases.
选择反应监测 (SRM) 使用灵敏和特异的质谱分析方法来测量多个样本中的目标分析物，但由于每个蛋白质的分析方法开发过程繁琐，因此尚未在蛋白质组学中广泛应用。我们描述了一种基于粗合成肽文库的高通量 SRM 分析方法的开发方法。我们通过生成和应用经过验证的酵母所有激酶和磷酸酶的 SRM 分析方法来说明该方法的强大功能。
