Zhu N H, Weiss H R
Department of Physiology and Biophysics, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
Am J Physiol. 1991 Feb;260(2 Pt 2):H626-31. doi: 10.1152/ajpheart.1991.260.2.H626.
A four-wavelength microspectrophotometric method has been developed to determine both O2 and CO saturation of hemoglobin in frozen small vessels. This method compensates for the nonspecific light loss related to the ice crystals and in addition can simultaneously measure both hemoglobin O2 and CO saturation in frozen blood. By using four wavelengths in the 540-580 nm range, it is possible to determine both O2 and CO saturations in frozen blood. This four-wavelength method demonstrated an accuracy of 6-8% within 95% confidence limits (SE of estimate = 3-4%) for both CO and O2 saturation determinations in frozen blood. The linear regression of the hemoglobin O2 saturation calibration was significant with an r2 value of 0.989. The linear regression of the CO saturation calibration was also significant with an r2 of 0.996. The repeatability of each measurement was on the order of 1-6%. This method also represents an accuracy within 5% for measurements in the frozen rabbit heart for both CO and O2 in vessels between 20 and 100 microns in diameter. It compares favorably with other previously reported methods. It shows a similar accuracy but requires fewer repeat measurements than the three-wavelength method. This method can provide accurate measurement of O2 and CO saturation in small frozen arterioles and venules of any organ.
已开发出一种四波长显微分光光度法，用于测定冷冻小血管中血红蛋白的氧饱和度和一氧化碳饱和度。该方法可补偿与冰晶相关的非特异性光损失，此外还能同时测量冷冻血液中血红蛋白的氧饱和度和一氧化碳饱和度。通过使用540 - 580纳米范围内的四个波长，可以测定冷冻血液中的氧饱和度和一氧化碳饱和度。这种四波长方法在95%置信限内对冷冻血液中一氧化碳和氧饱和度测定的准确度为6 - 8%（估计标准误差 = 3 - 4%）。血红蛋白氧饱和度校准的线性回归显著，r2值为0.989。一氧化碳饱和度校准的线性回归也显著，r2为0.996。每次测量的重复性约为1 - 6%。对于直径在20至100微米之间的血管中的一氧化碳和氧，该方法在冷冻兔心脏中的测量准确度也在5%以内。与其他先前报道的方法相比，它具有优势。它显示出相似的准确度，但比三波长方法所需的重复测量次数更少。该方法可以准确测量任何器官冷冻小动脉和小静脉中的氧饱和度和一氧化碳饱和度。
