Buschini M L T, Rigon J, Cordeiro J
Departamento de Biologia, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
Braz J Biol. 2009 Nov;69(4):1187-94. doi: 10.1590/s1519-69842009000500025.
This study was carried out in the Parque Municipal das Araucárias, located in the municipality of Guarapuava (PR) from May (06) to April (07). Its aim was to investigate which plants are used by Megachile (Moureapis) sp in provisioning of larvae, and to verify if this bee is oligolectic or polilectic. The collection of the plants was carried out during the bees activity period and in an area covering a 500 m radius from where the nests had been built. A sample of pollens from founded nests and from flowers was used to make permanent slides using the acetolysis method and with the aid of a light microscope, all pollen grains were examined in order to identify the plants used by this bee. A total of 80 pollen grain slides, from 16 nests were analysed. Although 99 plants were collected close to the bee's nesting site, in nest slides, the pollen of Ludwigia peruviana (36%) and Ludwigia sericea (63.6%) (Onagraceae) accounted for 99.6% of the total amount of pollen collected. With these results we are able to conclude that Megachile (Moureapis) sp is an oligolectic species and that these plants species are their important pollen source.
本研究于5月（06年）至4月（07年）在位于瓜拉普瓦瓦市（巴拉那州）的阿劳卡里亚斯市立公园进行。其目的是调查大切叶蜂（Moureapis）属的某种蜜蜂在为幼虫准备食物时使用哪些植物，并验证这种蜜蜂是寡食性还是多食性。植物采集在蜜蜂活动期间进行，采集区域为距蜂巢半径500米的范围。从已筑巢的蜂巢和花朵中采集花粉样本，采用醋酸分解法并借助光学显微镜制作永久玻片，对所有花粉粒进行检查以确定这种蜜蜂所使用的植物。共分析了来自16个蜂巢的80个花粉粒玻片。尽管在蜜蜂筑巢地点附近采集到了99种植物，但在蜂巢玻片上，秘鲁丁香蓼（36%）和绢毛丁香蓼（63.6%）（柳叶菜科）的花粉占所采集花粉总量的99.6%。根据这些结果，我们能够得出结论，大切叶蜂（Moureapis）属的某种蜜蜂是寡食性物种，并且这些植物物种是它们重要的花粉来源。
