Department of Applied Chemistry, Nanjing Agricultural University, China.
Arch Environ Contam Toxicol. 2010 Jul;59(1):100-8. doi: 10.1007/s00244-009-9426-4. Epub 2009 Dec 5.
Napropamide is a widely used herbicide for controlling weeds in crop production. However, extensive use of the herbicide has led to its accumulation in ecosystems, thus causing toxicity to crops and reducing crop production and quality. Salicylic acid (SA) plays multiple roles in regulating plant adaptive responses to biotic and environmental stresses. However, whether SA regulates plant response to herbicides (or pesticides) was unknown. In this study, we investigated the effect of SA on herbicide napropamide accumulation and biological processes in rapeseed (Brassica napus). Plants exposed to 8 mg kg(-1) napropamide showed growth stunt and oxidative damage. Treatment with 0.1 mM SA improved growth and reduced napropamide levels in plants. Treatment with SA also decreased the abundance of O (2) (-.) and H(2)O(2) as well as activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and increased activities of guaiacol peroxidase (POD) and glutathione-S-transferase (GST) in napropamide-exposed plants. Analysis of SOD, CAT, and POD activities using nondenaturing polyacrylamide gel electrophoresis (PAGE) confirmed the results. These results may help to understand how SA regulates plant response to organic contaminants and provide a basis to control herbicide/pesticide contamination in crop production.
萘丙酰胺是一种广泛用于作物生产中除草的除草剂。然而，除草剂的大量使用导致其在生态系统中积累，从而对作物产生毒性，降低作物产量和质量。水杨酸（SA）在调节植物对生物和环境胁迫的适应性反应中发挥多种作用。然而，SA 是否调节植物对除草剂（或农药）的反应尚不清楚。在这项研究中，我们研究了 SA 对油菜（甘蓝型油菜）中除草剂萘丙酰胺积累和生物过程的影响。暴露于 8 mg kg(-1)萘丙酰胺的植物表现出生长迟缓和氧化损伤。用 0.1 mM SA 处理可改善生长并降低植物中的萘丙酰胺水平。SA 处理还降低了 O(2) (-.) 和 H(2)O(2)的丰度以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）的活性，并增加了苯丙氨酸解氨酶（POD）和谷胱甘肽-S-转移酶（GST）在暴露于萘丙酰胺的植物中的活性。使用非变性聚丙烯酰胺凝胶电泳（PAGE）分析 SOD、CAT 和 POD 活性证实了这一结果。这些结果可能有助于了解 SA 如何调节植物对有机污染物的反应，并为控制作物生产中除草剂/农药污染提供依据。
