Department of Chemistry, KAIST, Daejeon, 305-701, Korea.
Chemistry. 2010 Jan 25;16(4):1351-5. doi: 10.1002/chem.200901708.
We report a new type of molecular sensor using a Au nanowire (NW)-Au nanoparticles (NPs) conjugated system. The Au NW-NPs structure is fabricated by the self-assembly of biotinylated Au NPs on a biotinylated Au NW through avidin; this creates hot spots between NW and NPs that strongly enhance the Raman signal. The number of the Au NPs attached to the NW is reproducibly proportional to the concentration of the avidin, and is also proportional to the measured surface-enhanced Raman scattering (SERS) signals. Since this well-defined NW-NPs conjugated sensor is only a few micrometer long, we expect that development of multiplex nanobiosensor of a few tens micrometer size would become feasible by combining individually modified multiple Au NWs together on one substrate.
我们报告了一种新型的分子传感器，使用了 Au 纳米线 (NW)-Au 纳米粒子 (NPs) 连接系统。Au NW-NPs 结构是通过生物素化的 Au NPs 通过亲和素自组装在生物素化的 Au NW 上形成的；这在 NW 和 NPs 之间产生了热点，强烈增强了拉曼信号。附着在 NW 上的 Au NPs 的数量与亲和素的浓度呈可重现的比例关系，并且与测量的表面增强拉曼散射 (SERS) 信号呈比例关系。由于这种定义明确的 NW-NPs 连接传感器只有几微米长，我们预计通过将单独修饰的多个 Au NW 组合在一个基底上，可以实现数十微米大小的多路复用纳米生物传感器的发展。
