Department of Plastic and Reconstructive Surgery, Dicle University, Diyarbakir, Turkey.
Microsurgery. 2010;30(1):55-60. doi: 10.1002/micr.20719.
This experimental study was designed to investigate and compare the effects of different anesthesia techniques on rat cremaster muscle flap microcirculation.
Fifty male Sprague-Dawley rats (130-150 g body weight) were divided into five experimental groups containing ten animals each. Group I, group II, and group III were designated as inhalation, epidural, and spinal anesthesia groups, respectively. Group IV was designated as a combination group for inhalation and epidural anesthesia. Group V was a combination group of inhalation and spinal anesthesia.
Group III and group V showed significant increases in the number of rolling and sticking leucocytes and in RBC volume (peripheral stasis) when compared with group I. Blood flow and velocity significantly increased without peripheral stasis in groups II and IV when compared with group I. Although there was no statistically significant difference in the numbers of rolling, sticking, and transmigrating leucocytes or in functional capillary perfusion, group IV had better flow hemodynamics in the peripheral microcirculation when compared with group I.
The inhalation and epidural anesthesia combination was determined to be the ideal anesthesia technique for improved peripheral microcirculation. Spinal anesthesia, either separately or in combination with inhalation anesthesia, has adverse effects on microcirculation.
本实验研究旨在探讨和比较不同麻醉技术对大鼠提睾肌皮瓣微循环的影响。
50 只雄性 Sprague-Dawley 大鼠（体重 130-150g）分为 5 个实验组，每组 10 只。第 I 组、第 II 组和第 III 组分别为吸入麻醉组、硬膜外麻醉组和脊髓麻醉组。第 IV 组为吸入麻醉和硬膜外麻醉联合组。第 V 组为吸入麻醉和脊髓麻醉联合组。
与第 I 组相比，第 III 组和第 V 组的滚动和黏附白细胞数量以及 RBC 体积（外周淤血）明显增加。与第 I 组相比，第 II 组和第 IV 组的血流和速度显著增加，无外周淤血。尽管滚动、黏附和迁移白细胞的数量或功能毛细血管灌注无统计学差异，但与第 I 组相比，第 IV 组的外周微循环血流动力学更好。
吸入麻醉和硬膜外麻醉联合被确定为改善外周微循环的理想麻醉技术。单独使用或与吸入麻醉联合使用脊髓麻醉会对微循环产生不良影响。
