Hasegawa M, Rosenbluth J
Department of Physiology, New York University School of Medicine, New York.
Anat Rec. 1991 Jan;229(1):138-43. doi: 10.1002/ar.1092290116.
Minced and triturated fragments from the spinal cord of normal rat fetuses (15-18 days gestation) labeled with the fluorescent dye fast blue (FB) were successfully transplanted into juvenile myelin-deficient rat spinal cord under direct observation. Clusters of myelinated fibers were found subsequently in the recipient spinal cord, and, by fluorescence microscopy, clusters of FB-labeled cells were found at corresponding sites. The results indicate that the surgical approach used is suitable for transplantation of tissue fragments into a defined region of juvenile rat spinal cord, that FB can be used to locate the transplanted cells subsequently, and that FB does not interfere with maturation of the donor glia or with myelin formation.
在直接观察下，将用荧光染料快蓝（FB）标记的正常大鼠胎儿（妊娠15 - 18天）脊髓的切碎和研磨碎片成功移植到幼年髓磷脂缺乏大鼠的脊髓中。随后在受体脊髓中发现了成束的有髓纤维，并且通过荧光显微镜检查，在相应部位发现了FB标记的细胞簇。结果表明，所采用的手术方法适用于将组织碎片移植到幼年大鼠脊髓的特定区域，FB可用于随后定位移植的细胞，并且FB不会干扰供体神经胶质细胞的成熟或髓磷脂的形成。
