急性淋巴细胞白血病患者的FLT3突变及其临床意义
[FLT3 mutation in patients with acute lymphoblastic leukemia and its clinical significance].
作者信息
Zhao Xin, Tang Ke-Jing, Tian Zheng, Chen Li-Ping, Mi Ying-Chang, Wang Jian-Xiang
机构信息
State Key Laboratory of Experimental Hematology, Department of Hematologic Oncology, Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2009 Oct;31(5):522-6.
OBJECTIVE
To investigate fms-like tyrosine kinase 3 (FLT3) mutation in patients with acute lymphoblastic leukemia (ALL) and its clinical significance.
METHODS
FLT3 mutation-internal tandem duplication (FLT3-ITD) and FLT3 mutation in the tyrosine kinase domain (FLT3-TKD) were detected using polymerase chain reaction (PCR) in the genomic DNA of 61 ALL patients and 7 healthy volunteers (as reference group). The PCR products of these patients who were detected with FLT3-ITD or FLT3-TKD were sent for sequence analysis.
RESULTS
In all these 61 ALL patients, FLT3-ITDs were identified in 2 patients and FLT3-TKD were identified in one patient.
CONCLUSION
FLT3-ITD and FLT3-TKD exist in a small proportion of ALL patients.
目的
探讨急性淋巴细胞白血病（ALL）患者中fms样酪氨酸激酶3（FLT3）突变情况及其临床意义。
方法
采用聚合酶链反应（PCR）检测61例ALL患者及7名健康志愿者（作为参照组）基因组DNA中的FLT3突变 - 内部串联重复（FLT3-ITD）和酪氨酸激酶结构域中的FLT3突变（FLT3-TKD）。对检测出FLT3-ITD或FLT3-TKD的患者的PCR产物进行测序分析。
结果
在这61例ALL患者中，2例患者检测到FLT3-ITD，1例患者检测到FLT3-TKD。
结论
一小部分ALL患者中存在FLT3-ITD和FLT3-TKD。
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