荧光原位杂交和流式细胞术在骨髓衰竭综合征中国患者端粒长度测量中的应用
[Application of fluorescence in situ hybridization and flow cytometry in the measurement of telomere length in Chinese patients with bone marrow failure syndrome].
作者信息
Liu Bo, Cui Wei, Han Bing, Wang Xuan, Lin Jie, Zhao Yong-Qiang
机构信息
Department of Hematology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2009 Oct;31(5):547-50.
OBJECTIVE
To measure the telomere length in patients with bone marrow failure syndrome (BMF) using fluorescence in situ hybridization and flow cytometry (Flow-FISH).
METHODS
Blood samples were collected from 8 patients with BMF. Telomere lengths of mononuclear cells from 8 BMF patients and granulocytes from 3 of these 8 BMF patients (all these 3 patients were suffered from aplastic anemia) were measured using Flow-FISH, and the results were compared with those of normal controls. Telomere lengths were measured by Southern blot in 3 patients, and the results were compared with those obtained from Flow-FISH.
RESULTS
Among these 8 BMF patients, 5 have decreased telomere length in their mononuclear cells. However, different white cell subgroups, specifically mononucleated cells and granulocytes, have different degree of telomere shortening. In 3 BMF patients who underwent both Flow-FISH and Southern blot, 2 patients had consistent measurement results.
CONCLUSION
Flow-FISH can be used for the measurement of telomere length in Chinese patients with BMF.
目的
采用荧光原位杂交和流式细胞术（Flow-FISH）检测骨髓衰竭综合征（BMF）患者的端粒长度。
方法
采集8例BMF患者的血样。使用Flow-FISH检测8例BMF患者单个核细胞以及其中3例（这3例均为再生障碍性贫血患者）粒细胞的端粒长度，并将结果与正常对照进行比较。对3例患者采用Southern印迹法测量端粒长度，并将结果与Flow-FISH获得的结果进行比较。
结果
在这8例BMF患者中，5例患者的单个核细胞端粒长度缩短。然而，不同的白细胞亚群，特别是单个核细胞和粒细胞，端粒缩短程度不同。在3例同时接受Flow-FISH和Southern印迹法检测的BMF患者中，2例患者的测量结果一致。
结论
Flow-FISH可用于检测中国BMF患者的端粒长度。
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