Lu Na, Hu Wei-ping, Deng Jian-cai, Zhai Shu-hua, Chen Xiao-min, Zhou Xiao-ping
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Huan Jing Ke Xue. 2009 Oct 15;30(10):2898-903.
Based on different ecological zone of Taihu Lake, alkaline phosphatase activity (APA), the kinetic parameters and the chemical parameters in water column from different zone of Taihu Lake were monitored, and the spatial distribution characteristics and the effects of environmental factors on the values of APA, Vmax and Km were studied. The results showed that the values of APA, Vmax and Km in water column from Taihu Lake had a spatial heterogeneous distribution. The spatial distribution characteristic of APA values was the same as that of Vmax, ones in water from different zones of Taihu Lake, namely, the maximal values of APA (9.43 +/- 5.30) nmol x (L x min)(-1) and Vmax (13.70 +/- 7.42) nmol x (L x min)(-1) occurred in water from estuary zone in western bank of Taihu Lake. The value distribution of APA and Vmax in other zone of Taihu Lake followed as: the central zone of Taihu Lake > the grass type zone of Taihu Lake > the Meiliang Bay zone of Taihu Lake > the Zhushan Bay zone of Taihu Lake > the Gonghu Bay zone of Taihu Lake. The value of Km from the grass type zone of Taihu Lake was the highest (20.50 +/- 11.30) micromol x L(-1) , and the one from estuary zone in western bank of Taihu Lake was the lowest (9.17 +/- 3.46) micromol x L(-1) The value of kinetic parameter Vmax was significantly positively correlated with the values of pH, total phosphorus (TP) and the chlorophyll a (Chla), with r(pH) = 0.6512** (p < 0.01), r(TP) = 0.4885** (p < 0.01) and r(Chla) = 0.7656** (p < 0.01), respectively. However, the effects of hydro-temperature, dissolved total phosphorus (DTP) and orthophosphorus (PO4(3-) -P) on Vmax values were negligible. There was no significant influence of the hydro-temperature, pH, DTP, PO4(3-)-P and Chla concentrations on the Km values, nevertheless significant negative relationship between the Km value and TP content was found with r = -0.3834* (p = 0.048).
基于太湖不同生态区，对太湖不同区域水柱中的碱性磷酸酶活性（APA）、动力学参数及化学参数进行了监测，并研究了APA、Vmax和Km值的空间分布特征以及环境因素对其的影响。结果表明，太湖水柱中APA、Vmax和Km值呈现空间异质性分布。APA值的空间分布特征与Vmax相同，太湖不同区域水体中的情况为：APA最大值（9.43±5.30）nmol·(L·min)⁻¹和Vmax最大值（13.70±7.42）nmol·(L·min)⁻¹出现在太湖西岸河口区水体中。太湖其他区域APA和Vmax值分布依次为：太湖中心区＞太湖草型区＞太湖梅梁湾区＞太湖竺山湾区＞太湖贡湾区。太湖草型区的Km值最高（20.50±11.30）μmol·L⁻¹，太湖西岸河口区的最低（9.17±3.46）μmol·L⁻¹。动力学参数Vmax值与pH值、总磷（TP）和叶绿素a（Chla）值呈显著正相关，相关系数分别为r(pH)=0.6512**（p＜0.01），r(TP)=0.4885**（p＜0.01）和r(Chla)=0.7656**（p＜0.01）。然而，水温、溶解性总磷（DTP）和正磷酸盐（PO₄³⁻-P）对Vmax值的影响可忽略不计。水温、pH值、DTP、PO₄³⁻-P和Chla浓度对Km值无显著影响，但发现Km值与TP含量呈显著负相关，r=-0.3834*（p=0.048）。
