Yin Gang, Hou Chunlin, Liu Wanshun, Han Baoqin, Wei Changzheng, Liu Shuangli, Guo Xingfeng, Yu Bing
Department of Orthopaedics, Changzheng Hospital of the Second Military Medical University, Shanghai 200003, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Nov;23(11):1347-9.
To study hemostasis of a new chitosan hemostatic powder.
Twenty-four adult SD rats were made the models of liver injury, male or female, and weighing 210-240 g. They were divided into three groups randomly (n = 8) depending on different hemostatic powders. The incision of the liver was treated with 300 mg Yunnan baiyao (group A1), chitosan hemostatic powder of pH 6.5 (group B1) and pH 7.5 (group C1), respectively. The bleeding time and bleeding amount were recorded. In vitro, with the modified Ree-White method, 2 mL artery blood from New Zealand white rabbit was added into the 0.2 mL solution of Yunnan baiyao, chitosan hemostatic powder of pH 6.5 and pH 7.5 (concentration of 0.2 mg/mL), respectively. The blood coagulation time was recorded. The chitosan blood clots of group B2 and group C2 were observed with scanning electron microscopy (SEM).
The bleeding time of group A1, group B1 and group C1 was (292 +/- 31), (261 +/- 23), and (224 +/- 28) s, respectively, the bleeding amount was (1.63 +/- 0.21), (1.47 +/- 0.18), and (1.18 +/- 0.17) g, respectively, showing statistically significant differences between groups B1, C1, and group A1 (P < 0.05), between group C1 and group B1 (P < 0.05). The blood clotting time of group A2, group B2, and group C2 was (653 +/- 41), (255 +/- 20), and (202 +/- 11) s, respectively, showing statistically significant differences between groups B2, C2, and group A2 (P < 0.05), between group C2 and group B2 (P < 0.05). The SEM showed that the blood cells of group B2 and group C2 gathered around the chitosan.
Chitosan hemostatic powder of pH 7.5 has good hemostasis.
研究一种新型壳聚糖止血粉的止血效果。
选取24只成年SD大鼠，雌雄不限，体重210 - 240 g，制作肝损伤模型。根据不同止血粉将其随机分为三组（n = 8）。分别用300 mg云南白药（A1组）、pH 6.5的壳聚糖止血粉（B1组）和pH 7.5的壳聚糖止血粉（C1组）处理肝脏切口，记录出血时间和出血量。体外实验采用改良的Ree-White法，分别向0.2 mL云南白药溶液、pH 6.5的壳聚糖止血粉溶液和pH 7.5的壳聚糖止血粉溶液（浓度均为0.2 mg/mL）中加入2 mL新西兰白兔动脉血，记录凝血时间。对B2组和C2组的壳聚糖血凝块进行扫描电子显微镜（SEM）观察。
A1组、B1组和C1组的出血时间分别为（292±31）、（261±23）和（224±28）s，出血量分别为（1.63±0.21）、（1.47±0.18）和（1.18±0.17）g，B1组、C1组与A1组之间差异有统计学意义（P < 0.05），C1组与B1组之间差异有统计学意义（P < 0.05）。A2组、B2组和C2组的凝血时间分别为（653±41）、（255±20）和（202±11）s，B2组、C2组与A2组之间差异有统计学意义（P < 0.05），C2组与B2组之间差异有统计学意义（P < 0.05）。扫描电子显微镜观察显示，B2组和C2组的血细胞聚集在壳聚糖周围。
pH 7.5的壳聚糖止血粉具有良好的止血效果。
