水飞蓟宾诱导人乳腺癌 MCF-7 细胞产生保护性超氧化物。
Silibinin induces protective superoxide generation in human breast cancer MCF-7 cells.
机构信息
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
出版信息
Free Radic Res. 2010 Jan;44(1):90-100. doi: 10.3109/10715760903300717.
The pharmacological activity of polyphenolic silibinin from milk thistle (Silybum marianum) is primarily due to its antioxidant property. However, this study found that silibinin promoted sustained superoxide (O(2)(.-)) production that was specifically scavenged by exogenous superoxide dismutase (SOD) in MCF-7 cells, while the activity of endogenous SOD was not changed by silibinin. Previous work proved that silibinin induced MCF-7 cell apoptosis through mitochondrial pathway and this study further proved that O(2)(.-) generation induced by silibinin was also related to mitochondria. It was found that respiratory chain complexes I, II and III were all involved in silibinin-induced O(2)(.-) generation. Moreover, it was found that silibinin-induced O(2)(.-) had protective effect, as exogenous SOD markedly enhanced silibinin-induced apoptosis.
水飞蓟宾（奶蓟草的主要活性多酚）的药理学活性主要归因于其抗氧化特性。然而，这项研究发现，水飞蓟宾可促进持续的超氧阴离子（O(2)(.-)）产生，而外源性超氧化物歧化酶（SOD）可特异性清除 MCF-7 细胞中的超氧阴离子（O(2)(.-)），同时水飞蓟宾不会改变内源性 SOD 的活性。先前的工作证明水飞蓟宾通过线粒体途径诱导 MCF-7 细胞凋亡，本研究进一步证明水飞蓟宾诱导的 O(2)(.-)生成也与线粒体有关。研究发现，呼吸链复合物 I、II 和 III 均参与了水飞蓟宾诱导的 O(2)(.-)生成。此外，还发现水飞蓟宾诱导的 O(2)(.-)具有保护作用，因为外源性 SOD 显著增强了水飞蓟宾诱导的细胞凋亡。
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