Lee Syng-Ook, Jeong Yun-Jeong, Im Hyo Gwon, Kim Cheorl-Ho, Chang Young-Chae, Lee In-Seon
Department of Food Science and Technology and The Center for Traditional Microorganism Resources (TMR), Keimyung University, Daegu 704-701, Republic of Korea.
Biochem Biophys Res Commun. 2007 Mar 2;354(1):165-71. doi: 10.1016/j.bbrc.2006.12.181. Epub 2007 Jan 2.
Matrix metalloproteinase-9 (MMP-9) plays an important role in the invasion and metastasis of cancer cells. In this study, we examined the inhibitory effect of silibinin, a flavonoid antioxidant from milk thistle (Silybum marianum L.) on PMA-induced MMP-9 expression in MCF-7 human breast carcinoma cells. Silibinin significantly and selectively suppressed PMA-induced MMP-9 expression in MCF-7. Silibinin has been found to inhibit PMA-induced MMP-9 gene transcriptional activity by blocking the activation of AP-1 via MAPK signaling pathways. Moreover, the Matrigel invasion assay showed that silibinin reduces PMA-induced invasion of MCF-7 cells. These results suggest that silibinin represents a potential anti-metastatic agent suppressing PMA-induced cancer cell invasion through the specific inhibition of AP-1-dependent MMP-9 gene expression.
基质金属蛋白酶-9（MMP-9）在癌细胞的侵袭和转移中起重要作用。在本研究中，我们检测了水飞蓟宾（一种来自水飞蓟的黄酮类抗氧化剂）对佛波酯（PMA）诱导的MCF-7人乳腺癌细胞中MMP-9表达的抑制作用。水飞蓟宾显著且选择性地抑制了PMA诱导的MCF-7细胞中MMP-9的表达。已发现水飞蓟宾通过阻断丝裂原活化蛋白激酶（MAPK）信号通路激活转录激活因子1（AP-1），从而抑制PMA诱导的MMP-9基因转录活性。此外，基质胶侵袭试验表明，水飞蓟宾可减少PMA诱导的MCF-7细胞侵袭。这些结果表明，水飞蓟宾是一种潜在的抗转移剂，通过特异性抑制AP-1依赖性MMP-9基因表达来抑制PMA诱导的癌细胞侵袭。
