Chemistry and Biochemistry Department, Utah State University, Logan, 84322-0300, USA.
J Cataract Refract Surg. 2009 Dec;35(12):2149-53. doi: 10.1016/j.jcrs.2009.06.030.
To quantitate hydroxyl radicals produced during phacoemulsification with various irrigating solutions and conditions used in cataract surgery.
Chemistry and Biochemistry Department, Utah State University, Logan, Utah, USA.
All experiments were performed using an Infiniti Vision System phacoemulsifier with irrigation and aspiration. Hydroxyl radicals were quantitated using electron spin resonance spectroscopy and a spectrophotometric assay for malondialdehyde, which is formed by the oxidation of deoxyribose by the hydroxyl radical.
Hydroxyl radical production increased during longitudinal-stroking phacoemulsification as power levels were increased in a nonlinear, nonexponential fashion. The detection of hydroxyl radical was reduced in irrigating solutions containing organic molecules (eg, citrate, acetate, glutathione, dextrose) and further reduced in Navstel, an irrigating solution containing a viscosity-modifying agent, hydroxypropyl methylcellulose.
Hydroxyl radicals produced in settings representative of those used in phacoemulsification cataract surgery were quantitated using the deoxyribose method. Hydroxyl radical production was dependent on the level of ultrasound power applied and the irrigating solution used. Oxidative stress on the eye during phacoemulsification may be minimized by using irrigating solutions that contain organic molecules, including the viscosity-modifying agent hydroxypropyl methylcellulose, that can compete for reaction with hydroxyl radicals.
定量分析白内障手术中不同灌流液和条件下超声乳化过程中产生的羟基自由基。
美国犹他州立大学化学与生物化学系。
所有实验均在配备灌流和抽吸系统的 Infiniti Vision System 超声乳化仪上进行。采用电子自旋共振波谱法和分光光度法测定丙二醛来定量检测羟基自由基，丙二醛是由羟基自由基氧化脱氧核糖形成的。
随着功率水平的非线性、非指数性增加，纵向超声乳化过程中羟基自由基的产生增加。在含有有机分子（如柠檬酸盐、醋酸盐、谷胱甘肽、葡萄糖）的灌流液中，羟基自由基的检测减少，而在含有粘度调节剂羟丙基甲基纤维素的 Navstel 灌流液中进一步减少。
使用脱氧核糖法对超声乳化白内障手术中代表性条件下产生的羟基自由基进行了定量检测。羟基自由基的产生取决于应用的超声功率水平和使用的灌流液。通过使用含有有机分子（包括粘度调节剂羟丙基甲基纤维素）的灌流液，可与羟基自由基竞争反应，从而减轻超声乳化过程中对眼睛的氧化应激。
