Schweiger A, Kostka G
Mol Biol Rep. 1977 Sep;3(5):353-9. doi: 10.1007/BF00420394.
Particles carrying heterogeneous nuclear RNA (30 S-particles) were prepared from rat liver and Zajdela hepatoma ascites cell nuclei after ultrasonic disruption. The ribonucleoprotein structures were disintegrated in the presence of 100mM spermidine. Using chromatography on Sepharose-polyadenylate a protein component has been obtained which possessed high affinity for heterogeneous nuclear RNA, polyuridylate and polyadenylate, and double-stranded DNA. This protein was the main species of the ribonucleoprotein studied; it showed bands with molcular weights of 37000 and 40000 respectively in SDS gel electrophoresis. The RNA-binding proteins isolated from liver and hepatoma had identical molecular weights and the same affinity for Sepharose-polyadenylate used in the isolation.
通过超声破碎从大鼠肝脏和Zajdela肝癌腹水细胞核中制备携带不均一核RNA的颗粒（30S颗粒）。在100mM亚精胺存在下，核糖核蛋白结构被分解。利用琼脂糖-聚腺苷酸柱层析，获得了一种对不均一核RNA、聚尿苷酸、聚腺苷酸和双链DNA具有高亲和力的蛋白质成分。该蛋白质是所研究的核糖核蛋白的主要种类；在SDS凝胶电泳中，它分别显示出分子量为37000和40000的条带。从肝脏和肝癌中分离出的RNA结合蛋白具有相同的分子量，并且对分离过程中使用的琼脂糖-聚腺苷酸具有相同的亲和力。
