Human Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, Brazil.
Cell Transplant. 2010;19(3):279-89. doi: 10.3727/096368909X481764. Epub 2009 Dec 8.
Adipose tissue may represent a potential source of adult stem cells for tissue engineering applications in veterinary medicine. It can be obtained in large quantities, under local anesthesia, and with minimal discomfort. In this study, canine adipose tissue was obtained by biopsy from subcutaneous adipose tissue or by suction-assisted lipectomy (i.e., liposuction). Adipose tissue was processed to obtain a fibroblast-like population of cells similar to human adipose-derived stem cells (hASCs). These canine adipose-derived stem cells (cASCs) can be maintained in vitro for extended periods with stable population doubling and low levels of senescence. Immunofluorescence and flow cytometry show that the majority of cASCs are of mesodermal or mesenchymal origin. cASCs are able to differentiate in vitro into adipogenic, chondrogenic, myogenic, and osteogenic cells in the presence of lineage-specific induction factors. In conclusion, like human lipoaspirate, canine adipose tissue may also contain multipotent cells and represent an important stem cell source both for veterinary cell therapy as well as preclinical studies.
脂肪组织可能代表了一种潜在的成体干细胞来源，可用于兽医组织工程应用。它可以在局部麻醉下，通过微创抽脂术（即吸脂术）从皮下脂肪组织中大量获取，而且患者的不适感极小。在这项研究中，通过活检从皮下脂肪组织或通过吸脂术（即抽脂术）获得犬脂肪组织。对脂肪组织进行处理，以获得类似于人脂肪来源干细胞（hASCs）的成纤维细胞样细胞群体。这些犬脂肪来源干细胞（cASCs）可以在体外长时间维持，具有稳定的倍增和低水平的衰老。免疫荧光和流式细胞术显示，大多数 cASCs 来源于中胚层或间充质。在存在谱系特异性诱导因子的情况下，cASCs 能够在体外分化为成脂细胞、成软骨细胞、成肌细胞和成骨细胞。总之，与人体脂肪抽吸物一样，犬脂肪组织也可能含有多能细胞，是兽医细胞治疗以及临床前研究的重要干细胞来源。
