Pansky Andreas, Roitzheim Barbara, Tobiasch Edda
Department of Natural Sciences, University of Applied Sciences Bonn-Rhein-Sieg, 53359 Rheinbach, Germany.
Clin Lab. 2007;53(1-2):81-4.
Human mesenchymal stem cells (HMSCs) which are isolated from bone marrow stroma, peripheral blood, dermis, muscle and adipose tissue have the advantage of potential autologous transplantation ability. They can be differentiated into chondrogenic, osteogenic, adipogenic and myogenic lineages. Problems of stem cells from bone marrow are low cell numbers, low isolated volumes, pain, and to some extent ethical concerns. The isolation of mesenchymal stem cells from human adipose tissue was recently identified as an alternative source, since these cells are easy to obtain in big cell numbers. Adipose tissue is derived from embryonic mesoderm and contains a heterogeneous stromal cell population. To achieve lineage-specific differentiation of these cells they have to be cultured in media supplemented with appropriate factors. Inductions of the cells into multiple mesenchymal lineages resulted in the expression of several lineage-specific genes, proteins and specific metabolic activity. In conclusion, the potential benefit of the multi-germline capacity of HMSCs seems to be a promising approach for allogenic cell therapy and human tissue engineering.
从骨髓基质、外周血、真皮、肌肉和脂肪组织中分离出的人间充质干细胞（HMSC）具有潜在的自体移植能力优势。它们可以分化为软骨生成、成骨、脂肪生成和肌生成谱系。来自骨髓的干细胞存在细胞数量少、分离量低、疼痛以及在一定程度上存在伦理问题。最近，从人脂肪组织中分离间充质干细胞被确定为一种替代来源，因为这些细胞易于大量获取。脂肪组织源自胚胎中胚层，包含异质性的基质细胞群体。为实现这些细胞的谱系特异性分化，必须在添加适当因子的培养基中培养它们。将细胞诱导分化为多个间充质谱系会导致几种谱系特异性基因、蛋白质的表达以及特定的代谢活性。总之，人间充质干细胞的多胚层分化能力的潜在益处似乎是同种异体细胞治疗和人体组织工程的一种有前景的方法。
