Pegoraro Adrian F, Ridsdale Andrew, Moffatt Douglas J, Pezacki John Paul, Thomas Brian K, Fu Libin, Dong Liang, Fermann Martin E, Stolow Albert
Department of Physics, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
Opt Express. 2009 Nov 9;17(23):20700-6. doi: 10.1364/OE.17.020700.
Using an all-fiber laser system consisting of a femtosecond Er/Yb fiber oscillator as the pump and an ultra-highly nonlinear fiber for Stokes generation, we demonstrate multimodal (TPF+SHG+CARS) non-linear optical microscopy of both tissue samples and live cells. Multimodal imaging was successfully performed with pixel dwell times as short as 4 micros at low laser powers (< 40 mW total).
我们使用一个全光纤激光系统,该系统由一个作为泵浦源的飞秒铒镱光纤振荡器和一根用于产生斯托克斯光的超高非线性光纤组成,展示了对组织样本和活细胞的多模态(双光子荧光+二次谐波产生+相干反斯托克斯拉曼散射)非线性光学显微镜成像。在低激光功率(总功率<40 mW)下,以低至4微秒的像素驻留时间成功实现了多模态成像。